Urinary Metabolomic Profile is Minimally Impacted by Common Storage Conditions and Additives.

IF 1.8 3区医学 Q3 OBSTETRICS & GYNECOLOGY International Urogynecology Journal Pub Date : 2025-02-24 DOI:10.1007/s00192-025-06069-2

Kelly C Weldon, Morgan Panitchpakdi, Andrés Mauricio Caraballo-Rodríguez, Alan J Wolfe, Pieter C Dorrestein, Linda Brubaker, Lindsey A Burnett

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Abstract

Background: Metabolomics reflects the molecular communications within biological systems. Urine is a noninvasive biofluid, rich in metabolites that serve as potential biomarkers for human health and disease. The impact of storage conditions and DNA stabilizers for urine samples in metabolomic studies remain unclear.

Objective: To evaluate the impact of common storage conditions and the presence of a DNA stabilizer, AssayAssure® (Thermo Scientific), on the metabolite content of voided human urine.

Methods: We assessed the urinary metabolite composition under different storage conditions and with the addition of AssayAssure® to determine its effect on metabolomic analysis.

Results: Urinary metabolite composition remained consistent across different storage conditions. However, the addition of AssayAssure® significantly altered the metabolic profile due to adduct formation. Despite these alterations, the identification of parent metabolites was not compromised, and biological differences were still distinguishable.

Conclusion: These findings suggest that urine biobanked under the tested storage conditions is suitable for metabolomic analysis. The addition of AssayAssure® does not hinder the detection of parent metabolites, although it may affect the overall metabolic profile.

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来源期刊

International Urogynecology Journal 医学-妇产科学

CiteScore

3.80

自引率

22.20%

发文量

406

审稿时长

3-6 weeks

期刊介绍： The International Urogynecology Journal is the official journal of the International Urogynecological Association (IUGA).The International Urogynecology Journal has evolved in response to a perceived need amongst the clinicians, scientists, and researchers active in the field of urogynecology and pelvic floor disorders. Gynecologists, urologists, physiotherapists, nurses and basic scientists require regular means of communication within this field of pelvic floor dysfunction to express new ideas and research, and to review clinical practice in the diagnosis and treatment of women with disorders of the pelvic floor. This Journal has adopted the peer review process for all original contributions and will maintain high standards with regard to the research published therein. The clinical approach to urogynecology and pelvic floor disorders will be emphasized with each issue containing clinically relevant material that will be immediately applicable for clinical medicine. This publication covers all aspects of the field in an interdisciplinary fashion