Moso bamboo sucrose synthase gene SUS3 promotes earlier germination of tiller buds in Dendrocalamus brandisii ‘Manxie Tianzhu’

IF 2.1 3区农林科学 Q2 FORESTRY Trees Pub Date : 2025-02-25 DOI:10.1007/s00468-025-02612-2

Weijie Sun, Muthusamy Ramakrishnan, Long-Hai Zou, Kai-Li Wang, Xiaohong Zhou, Mingbing Zhou

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Key message

Transient overexpression of PeSUS3 using a BaMV vector in D. brandisii ‘Manxie Tianzhu’ enhanced tillering bud germination and growth, suggesting a crucial role for sucrose metabolism in bamboo development.

Abstract

Bamboo shoots are highly sought after in China for their nutritional value. Dendrocalamus brandisii ‘Manxie Tianzhu’ (Manxie Sweet Dragon Bamboo) faces supply challenges due to limited distribution, necessitating faster growing, higher yielding varieties. The sucrose synthase gene, particularly PeSUS3, is vital for sucrose metabolism, nutrient transport, and regulating dormancy release and bud germination, yet its role in bamboo shoot production is not well understood. This study used a bamboo mosaic virus vector (BaMV) to overexpress PeSUS3 in D. brandisii ‘Manxie Tianzhu’ seedlings. PeSUS3 was cloned into the BaMV-P19-EGFP-2 vector, introduced into Agrobacterium tumefaciens, and used to infect tobacco leaves. The resulting virus was mechanically inoculated into the bamboo seedlings. Analysis revealed that PeSUS3 overexpression increased the number of tiller buds and accelerated germination by 1–2 days compared to controls. Despite the transient overexpression of PeSUS3, the increased sucrose content in the overexpressing plants suggests a role for sucrose metabolism in these processes. These findings emphasize the importance of the transient overexpression of PeSUS3 in promoting tillering bud germination and growth in D. brandisii ‘Manxie Tianzhu’, providing a foundation for future research on its function and mechanisms.

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毛竹蔗糖合成酶基因SUS3促进‘满泻天柱’分蘖芽提前萌发

关键信息利用BaMV载体瞬时过表达PeSUS3促进了竹的分蘖芽萌发和生长，提示蔗糖代谢在竹的发育过程中起重要作用。摘要竹笋因其营养价值在中国备受追捧。Manxie Tianzhu （Manxie甜龙竹）由于分布有限，面临供应挑战，需要生长更快、产量更高的品种。蔗糖合成酶基因，特别是PeSUS3，对蔗糖代谢、营养转运、调节休眠释放和芽萌发至关重要，但其在竹笋生产中的作用尚不清楚。本研究利用竹花叶病毒载体（BaMV）过表达PeSUS3，并将其应用于蔓泻天竹幼苗。将PeSUS3克隆到BaMV-P19-EGFP-2载体中，导入农杆菌感染烟叶。将得到的病毒机械接种到竹苗上。分析表明，与对照相比，PeSUS3过表达增加了分蘖芽的数量，加速了萌发1-2天。尽管PeSUS3短暂过表达，但在过表达植株中蔗糖含量的增加表明蔗糖代谢在这些过程中起作用。这些发现强调了PeSUS3瞬时过表达在促进“满榭天柱”分蘖芽萌发和生长中的重要性，为进一步研究其功能和机制奠定了基础。

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来源期刊

Trees 农林科学-林学

CiteScore

4.50

自引率

4.30%

发文量

113

审稿时长

3.8 months

期刊介绍： Trees - Structure and Function publishes original articles on the physiology, biochemistry, functional anatomy, structure and ecology of trees and other woody plants. Also presented are articles concerned with pathology and technological problems, when they contribute to the basic understanding of structure and function of trees. In addition to original articles and short communications, the journal publishes reviews on selected topics concerning the structure and function of trees.