Recombinant Humanized Collagen Enhances Secreted Protein Levels of Fibroblasts and Facilitates Rats' Skin Basement Membrane Reinforcement.

IF 5.2 3区医学 Q1 ENGINEERING, BIOMEDICAL Journal of Functional Biomaterials Pub Date : 2025-02-01 DOI:10.3390/jfb16020047

Shijia Ye, Boyu Chen, Lakshmi Jeevithan, Haoze Yang, Yaqi Kong, Xiaozhen Diao, Wenhui Wu

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Abstract

Collagen and its peptides exhibit remarkable antioxidant activity, superior biocompatibility, and water solubility, making them a significant research focus in skin care. Hence, the recombinant humanized collagen types I, III, and XVII complexed with niacinamide were developed to address damage in human foreskin fibroblasts (HFF-1) caused by ultraviolet radiation and to evaluate basement membrane proteins in a rat skin model. The Cell Counting Kit-8 (CCK-8) assay showed that higher concentrations of the complex increased the survival of damaged cells by approximately 10% and 22%, respectively, compared to the normal group after 16 and 48 h of treatment. Further biochemical analyses using ELISA and immunofluorescence (IF) confirmed that the complex enhanced the expression of collagen type IV, laminin, P63, and transforming growth factor-β (TGF-β) in the damaged cells. Additionally, the complex boosted the activity of the basement membrane in rat skin and stimulated the secretion of integrin, laminin, and perlecan. Overall, the recombinant humanized collagen complex effectively reinforced the skin's basement membrane.

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重组人源胶原蛋白提高成纤维细胞分泌蛋白水平，促进大鼠皮肤基底膜增强。

胶原蛋白及其多肽具有显著的抗氧化活性、优越的生物相容性和水溶性，是皮肤护理领域的重要研究热点。因此，我们开发了重组人源型胶原I、III和XVII与烟酰胺络合，以解决紫外线辐射引起的人包皮成纤维细胞（HFF-1）损伤，并在大鼠皮肤模型中评估基底膜蛋白。细胞计数试剂盒-8 （CCK-8）检测显示，与正常组相比，高浓度的复合物在处理16和48小时后，损伤细胞的存活率分别提高了约10%和22%。利用ELISA和免疫荧光（IF）进一步的生化分析证实，该复合物增强了受损细胞中IV型胶原、层粘连蛋白、P63和转化生长因子-β (TGF-β)的表达。此外，该复合物提高了大鼠皮肤基底膜的活性，刺激了整合素、层粘连蛋白和perlecan的分泌。总的来说，重组人源胶原复合物有效地增强了皮肤的基底膜。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Functional Biomaterials Engineering-Biomedical Engineering

CiteScore

4.60

自引率

4.20%

发文量

226

审稿时长

11 weeks

期刊介绍： Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.