The Results of FLASH Irradiation of Mice In Vivo with High-Energy Protons

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology Biophysics Pub Date : 2025-02-26 DOI:10.1134/S0006350924700842

A. E. Shemyakov, A. R. Dyukina, S. I. Zaichkina, A. V. Agapov, G. V. Mitsyn, K. N. Shipulin

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Abstract

The effect of high-energy (660 MeV) proton irradiation at the phasotron accelerator in FLASH mode (80 Gy/s) compared with the standard proton exposure power of 3.0 Gy/min was studied. When irradiated in two modes at doses of 1.0 and 1.5 Gy, the induction of cytogenetic damage in bone marrow cells and the state of lymphoid organs (thymus and spleen) were evaluated; survival under total in vivo irradiation of mice was analyzed at doses of 7.0 and 8.0 Gy. The growth rate of a model tumor under ex vivo irradiation was determined at doses of 40 and 60 Gy. It has been shown that irradiation of animals in the FLASH mode at a dose of 1.5 Gy protected the proliferative activity of the spleen and also led to a decrease in cytogenetic damage in bone marrow erythrocytes according to the micronucleus test compared with the standard irradiation mode at a dose of 1.5 Gy, that is, a milder effect of the FLASH mode dose was observed. However, irradiation of mice in FLASH mode at high doses (7.0 and 8.0 Gy) led to earlier death of animals compared to the standard irradiation regime. A tumor node formed with further growth only after FLASH irradiation of a suspension of Ehrlich ascites carcinoma at a dose of 40 Gy; in all other groups a tumor was not formed.

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高能质子FLASH照射小鼠体内的结果

研究了高速加速器在FLASH模式下高能（660 MeV）质子辐照（80 Gy/s）与标准质子辐照功率（3.0 Gy/min）的效果。在1.0 Gy和1.5 Gy两种照射模式下，观察骨髓细胞细胞遗传学损伤的诱导情况和淋巴器官（胸腺和脾脏）的状态；在7.0和8.0 Gy剂量下，分析小鼠在体内全辐射下的存活情况。在40和60 Gy的剂量下，测定模型肿瘤在离体照射下的生长速度。已有研究表明，与1.5 Gy的标准照射模式相比，1.5 Gy的FLASH模式照射动物可保护脾脏的增殖活性，并可导致骨髓红细胞细胞遗传学损伤的减少，即FLASH模式剂量的作用较轻。然而，与标准照射相比，在FLASH模式下以高剂量（7.0和8.0 Gy）照射小鼠导致动物更早死亡。40 Gy剂量的埃利希腹水癌悬浮液经FLASH照射后，肿瘤结形成并进一步生长；其他所有组均未形成肿瘤。

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.20

自引率

0.00%

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期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.