T. A. Efimov, A. I. Degtyarenko, R. V. Romashko, Y. N. Shkryl, M. N. Bezruk, I. M. Chernev, K. N. Galkin, D. V. Storozhenko
{"title":"Laser Micromechanical Sensor for Measurement of the Mass of Bacterial Cell Colonies","authors":"T. A. Efimov, A. I. Degtyarenko, R. V. Romashko, Y. N. Shkryl, M. N. Bezruk, I. M. Chernev, K. N. Galkin, D. V. Storozhenko","doi":"10.1134/S1062873824709759","DOIUrl":null,"url":null,"abstract":"<p>We presented results of applying an adaptive interferometry technique, based on dynamic holograms recorded in photorefractive crystal, to accurately determine the mass of biofilm bacteria colonies by the measuring of microcantilever frequency shift. During the experimental testing, biofilms of <i>Escherichia coli</i> were grown on the surface of a cantilever previously coated with a BaF<sub>2</sub> film. The proposed experimental setup is completely non-contact due to using an optical method of excitation and detection of cantilever oscillations. Based on the measured shift in the resonant frequency, the mass of the biofilm grown on the cantilever was calculated to be (2.072 ± 0.003) × 10<sup>–9</sup> gram.</p>","PeriodicalId":504,"journal":{"name":"Bulletin of the Russian Academy of Sciences: Physics","volume":"88 3 supplement","pages":"S322 - S324"},"PeriodicalIF":0.4800,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of the Russian Academy of Sciences: Physics","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1134/S1062873824709759","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Physics and Astronomy","Score":null,"Total":0}
引用次数: 0
Abstract
We presented results of applying an adaptive interferometry technique, based on dynamic holograms recorded in photorefractive crystal, to accurately determine the mass of biofilm bacteria colonies by the measuring of microcantilever frequency shift. During the experimental testing, biofilms of Escherichia coli were grown on the surface of a cantilever previously coated with a BaF2 film. The proposed experimental setup is completely non-contact due to using an optical method of excitation and detection of cantilever oscillations. Based on the measured shift in the resonant frequency, the mass of the biofilm grown on the cantilever was calculated to be (2.072 ± 0.003) × 10–9 gram.
期刊介绍:
Bulletin of the Russian Academy of Sciences: Physics is an international peer reviewed journal published with the participation of the Russian Academy of Sciences. It presents full-text articles (regular, letters to the editor, reviews) with the most recent results in miscellaneous fields of physics and astronomy: nuclear physics, cosmic rays, condensed matter physics, plasma physics, optics and photonics, nanotechnologies, solar and astrophysics, physical applications in material sciences, life sciences, etc. Bulletin of the Russian Academy of Sciences: Physics focuses on the most relevant multidisciplinary topics in natural sciences, both fundamental and applied. Manuscripts can be submitted in Russian and English languages and are subject to peer review. Accepted articles are usually combined in thematic issues on certain topics according to the journal editorial policy. Authors featured in the journal represent renowned scientific laboratories and institutes from different countries, including large international collaborations. There are globally recognized researchers among the authors: Nobel laureates and recipients of other awards, and members of national academies of sciences and international scientific societies.