New Insights on the miRNA Role in Diabetic Tendinopathy: Adipose-Derived Mesenchymal Stem Cell Conditioned Medium as a Potential Innovative Epigenetic-Based Therapy for Tendon Healing.

IF 4.8 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Biomolecules Pub Date : 2025-02-11 DOI:10.3390/biom15020264

Marina Russo, Caterina Claudia Lepre, Gianluca Conza, Nicoletta Tangredi, Giovanbattista D'Amico, Adriano Braile, Antimo Moretti, Umberto Tarantino, Francesca Gimigliano, Michele D'Amico, Maria Consiglia Trotta, Giuseppe Toro

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Abstract

Background: Adipose-derived mesenchymal stem cell conditioned medium (ASC-CM) improved the viability and wound closure of human tenocytes (HTCN) exposed to high glucose (HG) by activating the transforming growth factor beta 1 (TGF-β1) pathway.

Objectives: Since ASC-CM can also modulate microRNAs (miRNAs) in recipient cells, this study investigated the effects of ASC-CM on the miRNAs regulating tendon repair (miR-29a-3p, miR-210-3p and miR-21-5p) in HG-HTNC.

Methods: ASC-CM was obtained by ASCs isolated from the abdominal fat tissue of seven non-diabetic patients. HTNC were cultured in HG for 20 days, then scratched and exposed for 24 h to ASC-CM. qRT-PCR and ELISAs assessed miRNA and target levels.

Results: HG-HTNC exhibited a significant downregulation of miRNAs. ASC-CM restored the levels of miRNAs and their related targets involved in tendon repair.

Conclusions: The epigenetic modulation observed in HG-HTNC exposed to ASC-CM could be an innovative option in the management of diabetic tendinopathy.

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关于miRNA在糖尿病肌腱病变中的作用的新见解：脂肪源性间充质干细胞条件培养基作为肌腱愈合的潜在创新表观遗传学治疗方法。

背景：脂肪源性间充质干细胞条件培养基（ASC-CM）通过激活转化生长因子β1 （TGF-β1）通路，改善暴露于高糖（HG）的人腱细胞（HTCN）的生存能力和伤口愈合。目的：由于ASC-CM还可以调节受体细胞中的microrna (mirna)，本研究探讨了ASC-CM对HG-HTNC中调节肌腱修复的mirna （miR-29a-3p， miR-210-3p和miR-21-5p）的影响。方法：从7例非糖尿病患者腹部脂肪组织中分离ASCs获得ASC-CM。HTNC在HG中培养20 d，然后划伤，在ASC-CM中暴露24 h。qRT-PCR和elisa检测miRNA和靶蛋白水平。结果：HG-HTNC表现出明显的mirna下调。ASC-CM恢复了参与肌腱修复的mirna及其相关靶标的水平。结论：在暴露于ASC-CM的HG-HTNC中观察到的表观遗传调节可能是糖尿病肌腱病变管理的一种创新选择。

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来源期刊

Biomolecules Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

9.40

自引率

3.60%

发文量

1640

审稿时长

18.28 days

期刊介绍： Biomolecules (ISSN 2218-273X) is an international, peer-reviewed open access journal focusing on biogenic substances and their biological functions, structures, interactions with other molecules, and their microenvironment as well as biological systems. Biomolecules publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.