Description of Flavobacterium fructosi sp. nov., Flavobacterium xylosi sp. nov. and Flavobacterium zhouii sp. nov., three new members of the genus Flavobacterium.

IF 2 3区生物学 Q4 MICROBIOLOGY International journal of systematic and evolutionary microbiology Pub Date : 2025-02-01 DOI:10.1099/ijsem.0.006694

Dou Han, Lei-Lei Yang, Yu-Hua Xin, Qing Liu

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Abstract

Three novel strains, designated LB3P45^T, LS2P90^T and ZS1P70^T, were isolated from glaciers located on the Tibetan Plateau, PR China. These strains were Gram-stain-negative, aerobic, rod-shaped and yellow or orange coloured. Phylogenetic analysis based on the 16S rRNA gene and genomic sequences indicated that they belong to the genus Flavobacterium. The 16S rRNA gene sequence similarity among the three strains ranged from 97.4 to 98.6%. Strain LB3P45^T showed 98.9% and 98.7% similarity to Flavobacterium urumqiense CGMCC 1.9230^T and Flavobacterium xinjiangense JCM 11314^T, respectively. Strain LS2P90^T displayed 99.0% and 98.7% similarity to F. xinjiangense JCM 11314^T and F. urumqiense CGMCC 1.9230^T. Strain ZS1P70^T had the highest sequence similarity with F. xinjiangense JCM 11314^T (98.0%) and F. urumqiense CGMCC 1.9230^T (97.8%). The average nucleotide identity and digital DNA-DNA hybridization values between these strains and their closest relatives were lower than 93.0% and 47.9%, respectively. All three strains contained summed feature 3 (comprising C_16:1 ω7c and/or C_16:1 ω6c) as the major fatty acids. Based on phenotypic characteristics, phylogenetic analysis and genotypic data, the three novel species are proposed: Flavobacterium fructosi sp. nov. (LB3P45^T=CGMCC 1.11439^T=NBRC 114819^T), Flavobacterium xylosi sp. nov. (LS2P90^T=CGMCC 1.11685^T=NBRC 114823^T) and Flavobacterium zhouii sp. nov. (ZS1P70^T=CGMCC 1.24124^T=NBRC 114829^T).

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黄杆菌属新成员果黄杆菌、木黄杆菌和周黄杆菌的描述。

从青藏高原冰川中分离到3株新菌株LB3P45T、LS2P90T和ZS1P70T。这些菌株革兰氏染色阴性，好氧，杆状，黄色或橙色。基于16S rRNA基因和基因组序列的系统发育分析表明，它们属于黄杆菌属。3株的16S rRNA基因序列相似性在97.4 ~ 98.6%之间。菌株LB3P45T与乌鲁木齐黄杆菌CGMCC 1.9230T和新疆黄杆菌JCM 11314T的相似性分别为98.9%和98.7%。菌株LS2P90T与新疆F. JCM 11314T和乌鲁木齐F. CGMCC 1.9230T的相似性分别为99.0%和98.7%。菌株ZS1P70T与新疆镰刀菌JCM 11314T（98.0%）和乌鲁木齐镰刀菌CGMCC 1.9230T（97.8%）序列相似性最高。这些菌株与近缘菌株的平均核苷酸同源性和数字DNA-DNA杂交值分别低于93.0%和47.9%。3个菌株的主要脂肪酸均含有C16:1 ω7c和/或C16:1 ω6c的总特征3。根据表型特征、系统发育分析和基因型数据，提出了3个新种：果黄杆菌（LB3P45T=CGMCC 1.11439T=NBRC 114819T）、xylosi黄杆菌（LS2P90T=CGMCC 1.11685T=NBRC 114823T）和周黄杆菌（ZS1P70T=CGMCC 1.2424 24 t =NBRC 114829T）。

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.