Screening of Bacteria Promoting Carbon Fixation in Chlorella vulgaris Under High Concentration CO2 Stress.

IF 3.6 3区 生物学 Q1 BIOLOGY Biology-Basel Pub Date : 2025-02-03 DOI:10.3390/biology14020157
Chuntan Chen, Yu Wang, Qunwei Dai, Weiqi Du, Yulian Zhao, Qianxi Song
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Abstract

The cooperation between microalgae and bacteria can enhance the carbon fixation efficiency of microalgae. In this study, a microalgae-bacteria coexistence system under high-concentration CO2 stress was constructed, and the bacterial community structure of the entire system was analyzed using the 16S rDNA technique. Microbacterium sp., Bacillus sp., and Aeromonas sp. were screened and demonstrated to promote carbon fixation in Chlorella vulgaris HL 01 (C. vulgaris HL 01). Among them, the Aeromonas sp. + C. vulgaris HL 01 experimental group exhibited the most significant effect, with an increase of about 24% in the final biomass yield and a daily carbon fixation efficiency increase of about 245% (day 7) compared to the control group. Continuous cultivation of microalgae and bacterial symbiosis showed that bacteria could utilize the compounds secreted by microalgae for growth and could produce nutrients to maintain the vitality of microalgae. Detection of extracellular organic compounds of microorganisms in the culture broth by excitation-emission matrix spectral analysis revealed that bacteria utilized the aromatic proteinaceous compounds and others secreted by C. vulgaris HL 01 and produced new extracellular organic compounds required by C. vulgaris HL 01. The metabolic organic substances in the liquids of the experimental groups and the control group were analyzed by liquid chromatography-mass spectrometry, and it was found that 31 unique organic substances of C. vulgaris HL 01 were utilized by bacteria, and 136 new organic substances were produced. These differential compounds were mainly organic acids and their derivatives, benzene compounds, and organic heterocyclic compounds, etc. These results fully demonstrate that the carbon fixation ability and persistence of C. vulgaris HL 01 are improved through material exchange between microalgae and bacteria. This study establishes a method to screen carbon-fixing symbiotic bacteria and verifies that microalgae and bacteria can significantly improve the carbon fixation efficiency of microalgae for high-concentration CO2 through material exchange, providing a foundation for further research of microalgae-bacterial carbon fixation.

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Biology-Basel
Biology-Basel Biological Science-Biological Science
CiteScore
5.70
自引率
4.80%
发文量
1618
审稿时长
11 weeks
期刊介绍: Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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