{"title":"Isolation of <i>OsMetAP10</i>, a Peptidase_M24 Superfamily Gene, Regulating Heading Date in Rice.","authors":"Quanyi Sun, Jianhua Zhao, Guangda Wang, Yu Wang, Yuntao Zhu, Yu Yan, Zihang Chen, Zongxiang Chen, Zhiming Feng, Shimin Zuo","doi":"10.3390/biology14020178","DOIUrl":null,"url":null,"abstract":"<p><p>The heading date is one of the important traits in rice, which greatly affects grain yield and regional adaptability. Although the flowering pathways in rice have been extensively investigated, the genes involved in flowering remain largely unknown. Here, we report a rice <i>lhd</i> mutant, which showed late flowering under both long-day (LD) and short-day (SD) conditions. Through MutMap+ and linkage analysis, a deletion mutation in <i>OsMetAP10</i> was inferred as the most likely candidate for <i>lhd</i> late flowering. <i>OsMetAP10</i> encodes a methionine aminopeptidase that belongs to the peptidase_M24 subfamily III. The <i>OsMetAP10</i> gene is constitutively expressed in rice and is induced by light, with a rhythmic expression pattern. <i>OsMetAP10</i> knockout lines displayed late heading as the <i>lhd</i> mutation, while no alternations in morphology and heading were observed on <i>OsMetAP10</i> overexpression lines, further confirming the mutation of <i>OsMetAP10</i> as responsible for the late heading of <i>lhd</i>. Through RT-qPCR and transcriptome analysis, we revealed that the upregulated expression of the <i>FT-like</i> gene <i>OsFLT4</i>, a negatively flowering regulator, and the downregulation of flower development-related genes, <i>OsMADS14</i>, <i>OsMADS15</i>, and <i>OsMADS34</i>, played critical roles in determining the late flowering of the <i>OsMetAP10</i> mutation. This study reports a new gene affecting flowering and provides a new insight into the role of <i>OsMetAP10</i> in regulating rice heading.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 2","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11851548/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biology-Basel","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/biology14020178","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The heading date is one of the important traits in rice, which greatly affects grain yield and regional adaptability. Although the flowering pathways in rice have been extensively investigated, the genes involved in flowering remain largely unknown. Here, we report a rice lhd mutant, which showed late flowering under both long-day (LD) and short-day (SD) conditions. Through MutMap+ and linkage analysis, a deletion mutation in OsMetAP10 was inferred as the most likely candidate for lhd late flowering. OsMetAP10 encodes a methionine aminopeptidase that belongs to the peptidase_M24 subfamily III. The OsMetAP10 gene is constitutively expressed in rice and is induced by light, with a rhythmic expression pattern. OsMetAP10 knockout lines displayed late heading as the lhd mutation, while no alternations in morphology and heading were observed on OsMetAP10 overexpression lines, further confirming the mutation of OsMetAP10 as responsible for the late heading of lhd. Through RT-qPCR and transcriptome analysis, we revealed that the upregulated expression of the FT-like gene OsFLT4, a negatively flowering regulator, and the downregulation of flower development-related genes, OsMADS14, OsMADS15, and OsMADS34, played critical roles in determining the late flowering of the OsMetAP10 mutation. This study reports a new gene affecting flowering and provides a new insight into the role of OsMetAP10 in regulating rice heading.
期刊介绍:
Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.