Marisa de Oliveira Ribeiro, Mônica Barcellos Arruda, Alexandre Rodrigues Calazans, Alexandre Vicente Frederico, Anielly Ferreira Brito, Beatriz Vasconcello de Souza Barreto, Élida Millena de Vasconcelos Brandão, Hamilton Athayde, Kátia Cristina Silva Nascimento, Luiz Paulo de Brito Oliveira Souza, Pedro Henrique Cardoso, Priscilla Lopes da Silva Guimarães, Vanessa Duarte da Costa, Carlos Alexandre da Costa Silva, Alexandra Martins Soares, Josiane Iole, Guilherme Louzada, Luiz Amorim Filho, Patrícia Alvarez
{"title":"Detecting Arboviruses Through Screening Asymptomatic Blood Donors in Rio de Janeiro/Brazil During a Dengue Outbreak.","authors":"Marisa de Oliveira Ribeiro, Mônica Barcellos Arruda, Alexandre Rodrigues Calazans, Alexandre Vicente Frederico, Anielly Ferreira Brito, Beatriz Vasconcello de Souza Barreto, Élida Millena de Vasconcelos Brandão, Hamilton Athayde, Kátia Cristina Silva Nascimento, Luiz Paulo de Brito Oliveira Souza, Pedro Henrique Cardoso, Priscilla Lopes da Silva Guimarães, Vanessa Duarte da Costa, Carlos Alexandre da Costa Silva, Alexandra Martins Soares, Josiane Iole, Guilherme Louzada, Luiz Amorim Filho, Patrícia Alvarez","doi":"10.3390/v17020224","DOIUrl":null,"url":null,"abstract":"<p><p>Arthropod-borne viruses (arboviruses) dengue (DENV), chikungunya (CHIK), and Zika (ZIKV) have been responsible for a high number of outbreaks worldwide. However, their screening in blood donors is not mandatory, and asymptomatic cases might act as an important cause of virus transmission via transfusion. A study was conducted to assess the presence of DENV (serotypes 1-4), ZIKV, and CHIKV in pooled samples (pool size: six) from asymptomatic blood donors. A total of 9463 plasma pools, corresponding to 56,778 blood donations from asymptomatic blood donors who attended donor sessions at HEMORIO and other blood centers in Rio de Janeiro and Espírito Santo, was submitted to automated nucleic-acid extraction and PCR amplification using ZC D-<i>Tipagem</i> molecular assay (Bio-Manguinhos). In general, a pool prevalence of 1% (95/9463) and a donor prevalence of 0.17% (95/56,778) were observed. January and February 2024 had a total of 62 positive pools out of 95 (65.3%). Targets DENV-1 and -2 had a higher prevalence in the studied months-early summer-with 24 and 28 positive pools, respectively. ZC D-<i>Tipagem</i> molecular assay was able to detect the best-known arboviruses circulating in asymptomatic blood donors; this study suggested that ZIKV, CHIK, and DENV are circulating in asymptomatic blood donors before blood donations and can be transmitted to blood transfusion recipients.</p>","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"17 2","pages":""},"PeriodicalIF":3.5000,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11860395/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Viruses-Basel","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/v17020224","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VIROLOGY","Score":null,"Total":0}
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Abstract
Arthropod-borne viruses (arboviruses) dengue (DENV), chikungunya (CHIK), and Zika (ZIKV) have been responsible for a high number of outbreaks worldwide. However, their screening in blood donors is not mandatory, and asymptomatic cases might act as an important cause of virus transmission via transfusion. A study was conducted to assess the presence of DENV (serotypes 1-4), ZIKV, and CHIKV in pooled samples (pool size: six) from asymptomatic blood donors. A total of 9463 plasma pools, corresponding to 56,778 blood donations from asymptomatic blood donors who attended donor sessions at HEMORIO and other blood centers in Rio de Janeiro and Espírito Santo, was submitted to automated nucleic-acid extraction and PCR amplification using ZC D-Tipagem molecular assay (Bio-Manguinhos). In general, a pool prevalence of 1% (95/9463) and a donor prevalence of 0.17% (95/56,778) were observed. January and February 2024 had a total of 62 positive pools out of 95 (65.3%). Targets DENV-1 and -2 had a higher prevalence in the studied months-early summer-with 24 and 28 positive pools, respectively. ZC D-Tipagem molecular assay was able to detect the best-known arboviruses circulating in asymptomatic blood donors; this study suggested that ZIKV, CHIK, and DENV are circulating in asymptomatic blood donors before blood donations and can be transmitted to blood transfusion recipients.
期刊介绍：
Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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