The Genome Sequences of Baculoviruses from the Tufted Apple Bud Moth, Platynota idaeusalis, Reveal Recombination Between an Alphabaculovirus and a Betabaculovirus from the Same Host.

IF 3.5 3区医学 Q2 VIROLOGY Viruses-Basel Pub Date : 2025-01-30 DOI:10.3390/v17020202

Robert L Harrison, Michael A Jansen, Austin N Fife, Daniel L Rowley

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Abstract

The USDA-ARS collection of insect viruses at Beltsville, MD, USA, contains samples of an alphabaculovirus from larvae of the tufted apple bud moth, Platynota idaeusalis Walker, as well as a presumptive betabaculovirus from the same host species. The viruses in these samples-Platynota idaeusalis nucleopolyhedrovirus isolate 2680 (PlidNPV-2680) and Platynota idaeusalis granulovirus isolate 2683 (PlidGV-2683)-were characterized by electron microscopy of their occlusion bodies (OBs) and determination and analysis of their genome sequences. Scanning and transmission electron microscopy of the OBs revealed morphologies typical for alphabaculoviruses and betabaculoviruses. Sequencing viral DNA resulted in circular genomes of 121,881 bp and 106,633 bp for PlidNPV-2680 and PlidGV-2683, respectively. Similar numbers of ORFs (128 for PlidNPV-2680, 125 for PlidGV-2683) were annotated, along with ten homologous regions (hrs) in the PlidNPV-2680 genome and five intergenic regions of tandem direct repeats (drs) in the PlidGV genome. Phylogenetic inference from core gene alignments suggested that PlidMNPV-2680 represents a unique lineage within the genus Alphabaculovirus, while PlidGV-2683 was grouped with clade b betabaculoviruses. A comparison of the PlidNPV-2680 and PlidGV-2683 genomes revealed a 1516 bp region in PlidNPV-2680 that exhibited 97.5% sequence identity to a region of the PlidGV-2683 genome, suggesting that recombination had occurred recently between viruses from these lineages.

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丛状苹果芽蛾（Platynota idaeusalis）杆状病毒的基因组序列揭示了来自同一寄主的甲型杆状病毒和乙型杆状病毒之间的重组。

在美国马里兰州Beltsville， USDA-ARS收集的昆虫病毒中，含有来自簇状苹果芽蛾（Platynota ideaeusalis Walker）幼虫的甲型杆状病毒样本，以及来自同一宿主物种的推定乙型杆状病毒样本。用电镜观察和基因组序列分析方法对两种病毒——idea Platynota Platynota ideaeusalis nuclepolyhedrodrovirus 2680 （PlidNPV-2680）和颗粒病毒2683 （PlidGV-2683）进行了鉴定。OBs的扫描和透射电子显微镜显示了典型的甲型杆状病毒和乙型杆状病毒的形态。测序结果显示，PlidNPV-2680和PlidGV-2683的环状基因组长度分别为121,881 bp和106,633 bp。在PlidNPV-2680基因组中注释了相似数量的orf （PlidNPV-2680为128个，PlidGV-2683为125个），以及PlidNPV-2680基因组中的10个同源区域（hrs）和PlidGV基因组中的5个串联直接重复序列（drs）的基因间区域。核心基因序列的系统发育推断表明，PlidMNPV-2680在甲型杆状病毒属中具有独特的谱系，而PlidGV-2683属于乙型杆状病毒分支。对PlidNPV-2680和PlidGV-2683基因组的比较发现，PlidNPV-2680中有一个1516 bp的区域与PlidGV-2683基因组的一个区域具有97.5%的序列同源性，这表明来自这些谱系的病毒之间最近发生了重组。

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来源期刊

Viruses-Basel VIROLOGY-

CiteScore

7.30

自引率

12.80%

发文量

2445

审稿时长

1 months

期刊介绍： Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.