Mechanism of Bile-Processed Coptidis Rhizoma in the Treatment of Type 2 Diabetes Mellitus in Rats Based on Dissolution Kinetics and Untargeted Metabolomics

IF 1.7 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS Biomedical Chromatography Pub Date : 2025-02-28 DOI:10.1002/bmc.70040

Ying Zhu, Zhaowei Dong, Lu Yang, Qinwan Huang, Jin Wang

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Abstract

Bile-processed Coptidis Rhizoma (BPCR) exhibits stronger efficacy in treating T2DM than Coptidis Rhizoma(CR) alone. However, the synergistic mechanism of its processing remains unknown. This study utilized HPLC to determine the content and dissolution characteristics of alkaloid components in BPCR before and after processing. The results indicated that the diffusion of the alkaloids in BPCR is stronger than that of CR, and their dissolution conforms to the Weibull equation. Additionally, BPCR significantly reduced fasting blood glucose (FBG) and serum insulin (FINS) levels in T2DM rats induced by a high-fat diet (HFD) and streptozotocin (STZ), improved glucose and lipid metabolism, and mitigated liver damage. Serum metabolomics analysis based on UPLC-Q-TOF-MS revealed that BPCR significantly regulates 27 endogenous differential biomarkers. The underlying mechanism may be related to glycerophospholipid metabolism, linoleic acid metabolism, steroid biosynthesis, and arachidonic acid metabolism pathways.

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基于溶出动力学和非靶向代谢组学研究胆黄连治疗2型糖尿病的机制

胆汁黄连（BPCR）治疗2型糖尿病的疗效优于单用黄连（CR）。然而，其加工的协同机制尚不清楚。本研究采用高效液相色谱法测定了BPCR炮制前后生物碱组分的含量和溶出特性。结果表明，生物碱在BPCR中的扩散强于CR，其溶出符合Weibull方程。此外，BPCR可显著降低高脂饮食（HFD）和链脲佐菌素（STZ）诱导的T2DM大鼠的空腹血糖（FBG）和血清胰岛素（FINS）水平，改善糖脂代谢，减轻肝损伤。基于UPLC-Q-TOF-MS的血清代谢组学分析显示，BPCR显著调节27种内源性差异生物标志物。其潜在机制可能与甘油磷脂代谢、亚油酸代谢、类固醇生物合成和花生四烯酸代谢途径有关。

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.