TRIM44 facilitates aggressive behaviors in multiple myeloma through promoting ZEB1 deubiquitination.

IF 2.9 4区医学 Q3 ENDOCRINOLOGY & METABOLISM Discover. Oncology Pub Date : 2025-02-27 DOI:10.1007/s12672-025-01933-5

Hui Qi, Jing Wang, Lixia Cao

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Abstract

Background: Tripartite motif-containing 44 (TRIM44) involves in various tumor development. This study investigated role of TRIM44 in multiple myeloma (MM).

Materials and methods: TRIM44 levels in bone marrow tissues and MM cell lines was detected by quantitative reverse transcription PCR (RT-qPCR). Cell viability, migration, and invasion of MM cells were evaluated under the interference of TRIM44 expression. The role of TRIM44 on regulating tumor growth in vivo was also investigated in subcutaneous tumor xenograft models. The protein interact between TRIM44 and Zinc Finger E-Box Binding Homeobox 1 (ZEB1) was also studied according IP followed by western blotting assay.

Results: TRIM44 was all highly expressed in collected bone marrow tissues and MM cell lines. Cell viability, migration, and invasion of MM cells with low expression of TRIM44 was significantly inhibited. Over-expression of TRIM44 can down-regulate the ZEB1 ubiquitination to enhance the protein stability.

Conclusions: TRIM44 exerts as an oncogenic factor to induce the oncogenesis of MM by stabilizing ZEB1.

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TRIM44通过促进ZEB1的去泛素化促进多发性骨髓瘤的侵袭行为。

背景：Tripartite motif-containing 44 （TRIM44）参与多种肿瘤的发展。本研究探讨了TRIM44在多发性骨髓瘤（MM）中的作用。材料与方法：采用定量反转录PCR （RT-qPCR）检测骨髓组织和MM细胞株中TRIM44的水平。在TRIM44表达干扰下，评估MM细胞的活力、迁移和侵袭能力。在皮下肿瘤异种移植模型中也研究了TRIM44在体内调节肿瘤生长的作用。采用免疫印迹法研究TRIM44与锌指E-Box Binding Homeobox 1 （ZEB1）蛋白的相互作用。结果：TRIM44在采集的骨髓组织和MM细胞系中均有高表达。TRIM44低表达的MM细胞的细胞活力、迁移和侵袭均受到显著抑制。过表达TRIM44可下调ZEB1泛素化，增强蛋白稳定性。结论：TRIM44作为一种致癌因子，通过稳定ZEB1介导MM的癌变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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