Effect of narrowband ultraviolet B (311 nm) exposure on skin carcinogenesis in Wistar rats.

IF 1.5 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Journal of Advanced Veterinary and Animal Research Pub Date : 2024-12-29 eCollection Date: 2024-12-01 DOI:10.5455/javar.2024.k861

Roro Inge Ade Krisanti, Septelia Inawati Wanandi, Puspita Eka Wuyung, Aida S D Hoemardani

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Abstract

Objective: The aim of this study is to determine narrowband UVB (NB-UVB) irradiation's effect on the promotion of skin cancer, particularly its effect on DNA damage, oxidative stress, inflammation, and histological changes in Wistar rat skin.

Materials and methods: Wistar rats were selected for this study and randomly divided into control, dimethylbenzanthracene (DMBA), and DMBA+NB-UVB groups. The rats were given a single dose of DMBA and exposed to NB-UVB 3 times a week for 10 weeks. The radiation dose started with 1 minimal erythema dose, which is equivalent to 3.192 J/cm². In the 11th week, analysis on cyclobutene pyrimidine dimer (CPD), malondialdehyde (MDA), nuclear factor kappa-B (NFκB), inflammatory cytokines, and histopathology examination of the skin tissue was conducted.

Results: Higher CPD, MDA, NFκB, tumor necrosis factor a (TNF-a), interleukin (IL)-6, IL-11, IL-10, and IL-12 levels in rats exposed to DMBA+NB-UVB for 10 weeks compared to control and DMBA groups. Macroscopic examination presented erythema, skin thickening, desquamation, ulcer, and crust. Histopathology examination showed hyperkeratosis, acanthosis, atypical keratinocytes, irregular arrangement of the basement membrane, and inflammatory cell infiltration in the DMBA+NB-UVB group.

Conclusion: This research has shown that 10 weeks of a combination of DMBA and NB-UVB irradiation induced DNA damage, oxidative stress, inflammation, and histological changes in the Wistar rat skin.

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窄带紫外线B （311 nm）照射对Wistar大鼠皮肤癌变的影响。

研究目的本研究旨在确定窄带紫外线（NB-UVB）照射对皮肤癌的促进作用，尤其是对 Wistar 大鼠皮肤 DNA 损伤、氧化应激、炎症和组织学变化的影响：研究选取 Wistar 大鼠，随机分为对照组、二甲基苯并蒽（DMBA）组和 DMBA+NB-UVB 组。大鼠接受单剂量 DMBA 和每周 3 次的 NB-UVB 照射，为期 10 周。辐射剂量从 1 个最小红斑剂量开始，相当于 3.192 J/cm²。第 11 周，对环丁烯嘧啶二聚体（CPD）、丙二醛（MDA）、核因子卡巴-B（NFκB）、炎症细胞因子进行分析，并对皮肤组织进行组织病理学检查：结果：与对照组和 DMBA 组相比，暴露于 DMBA+NB-UVB 10 周的大鼠体内 CPD、MDA、NFκB、肿瘤坏死因子 a (TNF-a)、白细胞介素 (IL)-6、IL-11、IL-10 和 IL-12 水平较高。宏观检查显示大鼠皮肤出现红斑、增厚、脱屑、溃疡和结痂。组织病理学检查显示，DMBA+NB-UVB 组出现角化过度、棘层增生、非典型角质细胞、基底膜排列不规则和炎性细胞浸润：本研究表明，DMBA 和 NB-UVB 联合照射 10 周会诱发 Wistar 大鼠皮肤的 DNA 损伤、氧化应激、炎症和组织学变化。

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来源期刊

Journal of Advanced Veterinary and Animal Research Veterinary-Veterinary (all)

CiteScore

2.70

自引率

7.10%

发文量

审稿时长

6 weeks

期刊介绍： Journal of Advanced Veterinary and Animal Research (JAVAR) - is an open access, international, peer-reviewed, quarterly, highly-indexed scientific journal publishing original research findings and reviews on all aspects of veterinary and animal sciences. Basic and applied researches on- - Anatomy & histology - Animal health economics - Animal nutrition - Animal reproduction - Animal science - Antimicrobial resistance (AMR) - Biochemistry - Biotechnology - Dairy science - Epidemiology - Food hygiene and technology - Genetics and breeding - Immunology - Microbiology - Parasitology - Pathology - Pharmacology & toxicology - Physiology - Poultry science - Preventive veterinary medicine - Public health - Surgery & obstetrics - Veterinary extension studies - Wildlife & aquatic medicine - Zoo animal medicine.