New information on adults pentastomids (Crustacea: Pentastomida) found in ophidians from Argentina: Insights from 28S rDNA and COI mtDNA.

IF 1.2 4区医学 Q4 PARASITOLOGY Systematic Parasitology Pub Date : 2025-02-27 DOI:10.1007/s11230-025-10222-2

Martin Miguel Montes, Exequiel Oscar Furlan, Jorge Adrian Barneche, Clara Vercellini, Tomas Acuña Gonzalez, Marina Ibañez Shimabukuro, Vanesa Arzamendia

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Abstract

Pentastomids are a group of crustaceans adapted for parasitic life. In South America, twenty-five species have been recorded, with only nine species reported in Argentina. Only three studies have focused on the molecular analysis of pentastomids in South America. Therefore, the aim of this work is to report the sequences of two pentastomids found in Argentina. A "yarara grande" (Bothrops alternatus) and a common swamp snake (Erythrolamprus poecilogyrus) were found dead on roads in northeastern Argentina and examined for parasites. Pentastomids were found in the lungs, removed, and preserved in 96% ethanol for molecular analysis. DNA was extracted, and the 28S and COI genes were amplified. The genetic analysis revealed the presence of Porocephalus cf. crotali in B. alternatus and Kiricephalus cf. coarctatus in E. poecilogyrus. This study provides the first South American COI sequences for adults of these species. Additionally, we report the first 28S sequence for K. cf. coarctatus. Furthermore, these results suggest discordance between morphological classification and molecular data at the family and subfamily levels. Further research on genus morphology and more comprehensive genetic data are required to definitively resolve the taxonomic placement of these families and their constituent genera.

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阿根廷蛇属五角虫成虫（甲壳纲：Pentastomida）的新资料：来自28S rDNA和COI mtDNA的见解。

Pentastomids是一组适应寄生生活的甲壳类动物。在南美洲，有记录的有25种，而在阿根廷只有9种。只有三项研究集中在南美的五形虫的分子分析上。因此，本工作的目的是报道在阿根廷发现的两个五形体的序列。在阿根廷东北部的道路上发现了一条“大沼泽蛇”（Bothrops alternatus）和一条常见的沼泽蛇（Erythrolamprus poecilogyrus）的尸体，并进行了寄生虫检查。在肺中发现Pentastomids，将其去除，保存在96%的乙醇中进行分子分析。提取DNA，扩增28S和COI基因。遗传分析结果显示，在竹背蝽中存在crotali porcephalus，在拟南蝽中存在coarctatus Kiricephalus。本研究首次提供了这些物种成虫的南美COI序列。此外，我们还报道了K. cf. coarctatus的第一个28S序列。此外，这些结果表明在科和亚科水平上形态分类与分子数据之间存在不一致。为了确定这些科及其组成属的分类位置，需要进一步的属形态研究和更全面的遗传资料。

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来源期刊

Systematic Parasitology 医学-寄生虫学

CiteScore

2.20

自引率

23.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Systematic Parasitology publishes papers on the systematics, taxonomy and nomenclature of the following groups: Nematoda (including plant-parasitic), Monogenea, Digenea, Cestoda, Acanthocephala, Aspidogastrea, Cestodaria, Arthropoda (parasitic copepods, hymenopterans, mites, ticks, etc.), Protozoa (parasitic groups), and parasitic genera in other groups, such as Mollusca, Turbelleria, etc. Systematic Parasitology publishes fully illustrated research papers, brief communications, and fully illustrated major revisions. In order to maintain high standards, all contributors describing new taxa are asked to state clearly where the holotype is deposited and to make paratypes available for examination by the referees. It is recognized that, in some cases, this may cause problems for the authors, but it is hoped that by adhering to this rule authors may be protected against rapid synonymy of their taxa, and the types will be preserved for posterity.