Four distinct pathways involved in a “tug-of-war” lead to the non-linear nature of phenolic chemistry during lactic acid fermentation of fruits and vegetables

Abstract

Introduction: Lactic acid fermentation of fruits and vegetables (F&V) is endowed with new nutrients and flavors. “Phenolics” is a hot spot in this area, which evolve irregularly during fermentation. However, the mechanism about this non-linear phenomenon has been poorly understood. Objectives: This paper was aimed at decoding the mechanism about the non-linear nature of phenolic chemistry during lactic acid fermentation of F&V. Methods: Mango and cress slurries were fermented by Lactiplantibacillus plantarum. Different fractions of the slurry samples were analyzed comprehensively. Four pathways relating to phenolic changes were extracted, including adsorption of free phenolics by F&V cell wall materials, microbial adsorption and biotransformation of free phenolics, destabilization of covalent bond between bound phenolics and F&V cell walls. The in-depth features of each pathway during fermentation were explored by multidisciplinary methodologies. Results: Throughout both fermentation of mango and cress slurries, free phenolics and the F&V cell wall components undergo dynamic changes. Due to the reduction of pectin fraction in the F&V cell walls during fermentation, the adsorption of free phenolics by F&V cell wall materials through surface diffusion was improved. Also, microbial cells, especially in the latter stages of fermentation, were capable of adsorbing free phenolics through surface diffusion. Moreover, the padC and bglB genes encoding phenolic acid decarboxylase and β-glucosidase were expressed during fermentation, contributing to the conversion of free phenolics. Besides, bound phenolics were not released during fermentation, although its covalent was weakened. The features of the above pathways vary with the fermentation stages and the composition of F&V components, resulting in non-linear changes of free phenolics during F&V fermentation. Conclusion: F&V cell wall adsorption, microbial adsorption, microbial biotransformation, and destabilization of the covalent bond of bound phenolics compete in a dynamic “tug-of-war”, leading to non-linear nature of phenolic chemistry during F&V fermentation.