Recent advances on engineering Escherichia coli and Corynebacterium glutamicum for efficient production of L-threonine and its derivatives

Abstract

L-threonine, one of the three major amino acids, plays a vital role in various industries such as food, feed, pharmaceuticals, and cosmetics. Currently, the fermentation-based production of L-threonine has evolved into an efficient, cost-effective, and environmentally friendly industrial process. Escherichia coli and Corynebacterium glutamicum, as the industrial workhorses of amino acids production, have long been widely studied due to their well-established genetic backgrounds and powerful molecular tools. This review focuses on recent advances in the microbial production of L-threonine by metabolic engineering. From three key modules, including L-threonine synthesis module, central metabolism module and global regulation module, we provide a comprehensive analysis on the entire metabolic pathway of L-threonine and the global regulation of the production process. Furthermore, we systematically summarize biotransformation methods for producing high-value derivatives of L-threonine, thereby broadening the application scope and market potential of L-threonine. Overall, this review shows many effective strategies for the biosynthesis of L-threonine, and offers guidance for the microbial production of L-aspartate family amino acids and their derivatives.