Heiner Kolp , Jana K. Hackert , Marco Heerdegen , Christa Unger , Tina Sellmann , Katrin Porath , Valentin Neubert , Marco Weiergräber , Timo Kirschstein , Rüdiger Köhling
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引用次数: 0
Abstract
Objective
To study the effects of extracellular bromide in a novel immature rat pilocarpine model compared to the standard adolescent rat model.
Methods
We employed an immature rat model of repetitive pilocarpine-induced status epilepticus (340 mg/kg on postnatal days 9, 11 and 15). The electrophysiological characterization of the Schaffer collateral CA1-synapse and of CA1 pyramidal neurons was performed in 30–70 day-old animals. To explore the effects of bromide, 20 mM NaCl in the bath solution was replaced by 20 mM NaBr. We compared our findings in the immature model with data from the standard adolescent model of a single pilocarpine-induced status epilepticus (340 mg/kg on postnatal day 30) obtained from 40−90 day-old animals.
Results
In the immature, but not in the adolescent model, extracellular bromide (20 mM) enhanced the GABAA-receptor component of the inhibitory postsynaptic potential, hyperpolarized the GABAA-receptor reversal potential and reduced intrinsic excitability. However, bromide left high-frequency stimulation-induced long-term potentiation unaltered – in both the immature and the adolescent model.
Significance
The immature model, but not the commonly used adolescent pilocarpine model, showed a persistent bromide-enhanced GABAA-receptor function leading to reduced intrinsic excitability. Hence, we suggest that immature animal models are needed to explore novel therapeutic strategies for epilepsies acquired during infancy.
期刊介绍：
Epilepsy Research provides for publication of high quality articles in both basic and clinical epilepsy research, with a special emphasis on translational research that ultimately relates to epilepsy as a human condition. The journal is intended to provide a forum for reporting the best and most rigorous epilepsy research from all disciplines ranging from biophysics and molecular biology to epidemiological and psychosocial research. As such the journal will publish original papers relevant to epilepsy from any scientific discipline and also studies of a multidisciplinary nature. Clinical and experimental research papers adopting fresh conceptual approaches to the study of epilepsy and its treatment are encouraged. The overriding criteria for publication are novelty, significant clinical or experimental relevance, and interest to a multidisciplinary audience in the broad arena of epilepsy. Review articles focused on any topic of epilepsy research will also be considered, but only if they present an exceptionally clear synthesis of current knowledge and future directions of a research area, based on a critical assessment of the available data or on hypotheses that are likely to stimulate more critical thinking and further advances in an area of epilepsy research.
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