Protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo chorioallantoic membrane model.

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS STAR Protocols Pub Date : 2025-03-21 Epub Date: 2025-02-28 DOI:10.1016/j.xpro.2025.103663
Tommy Chastel, Serena Filiberti, Stefania Mitola, Roberto Ronca, Andrei Turtoi, Michela Corsini
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引用次数: 0

Abstract

The chick embryo chorioallantoic membrane (CAM) is a viable alternative in vivo model to explore cancer biology due to its simplicity, accessibility, and cost-effectiveness. Here, we present a protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo CAM model. We describe steps for initial general preparation and for the angiogenic and tumorigenic assays in two distinct sections.

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使用卵中鸡胚绒毛膜模型进行血管生成和肿瘤生成试验的规程。
鸡胚绒毛尿囊膜(CAM)由于其简单、易获得和成本效益,是一种可行的替代癌症生物学体内模型。在这里,我们提出了一种使用蛋内鸡胚胎CAM模型进行血管生成和致瘤性实验的方案。我们在两个不同的部分中描述了初始一般准备和血管生成和致瘤性测定的步骤。
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来源期刊
STAR Protocols
STAR Protocols Biochemistry, Genetics and Molecular Biology-General Biochemistry, Genetics and Molecular Biology
CiteScore
2.00
自引率
0.00%
发文量
789
审稿时长
10 weeks
期刊介绍:
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