Isolating Astrocyte-Derived Extracellular Vesicles From Urine.

IF 6.5 2区医学 Q1 NANOSCIENCE & NANOTECHNOLOGY International Journal of Nanomedicine Pub Date : 2025-02-26 eCollection Date: 2025-01-01 DOI:10.2147/IJN.S492381

Xin-Hui Xie, Mian-Mian Chen, Shu-Xian Xu, Junhua Mei, Qing Yang, Chao Wang, Honggang Lyu, Qian Gong, Zhongchun Liu

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Abstract

Introduction: Brain-derived extracellular vesicles (BDEVs) can cross the blood-brain barrier and enter the periphery. Therefore, quantifying and analyzing peripherally circulating BDEVs offer a promising approach to directly obtain a window into central nervous system (CNS) pathobiology in vivo. Rapidly evolving CNS diseases require high-frequency sampling, but daily venipuncture of human subjects is highly invasive and usually unfeasible.

Methods: To address this challenge, here we present a novel method for isolating astrocyte-derived extracellular vesicles from urine (uADEVs), combining urine concentration, ultracentrifugation to isolate total EVs, and then glutamate-aspartate transporter (GLAST) EV isolation using an anti-GLAST antibody.

Results: The identity of these GLAST+EVs as uADEVs was confirmed by transmission electron microscopy, nanoparticle tracking analysis, western blotting, and assessment of astrocyte-related neurotrophins.

Conclusions: Leveraging the convenience and availability of urine samples, the non-invasive uADEV approach provides a novel tool that allows high-frequency sampling to investigate rapidly evolving CNS diseases.

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从尿液中分离星形胶质细胞衍生的细胞外囊泡

脑源性细胞外囊泡（Brain-derived extracellular vesicles, BDEVs）可以穿过血脑屏障进入外周。因此，量化和分析外周循环BDEVs为直接获得体内中枢神经系统（CNS）病理生物学窗口提供了一种有希望的方法。快速发展的中枢神经系统疾病需要高频采样，但每天对人类受试者进行静脉穿刺是高度侵入性的，通常是不可行的。方法：为了解决这一挑战，我们提出了一种从尿液中分离星形胶质细胞来源的细胞外囊泡（uADEVs）的新方法，结合尿液浓度、超离心分离总EV，然后使用抗GLAST抗体分离谷氨酸-天冬氨酸转运体（GLAST） EV。结果：通过透射电镜、纳米颗粒跟踪分析、免疫印迹和星形胶质细胞相关神经营养因子的评估，证实了这些GLAST+ ev为uADEVs。结论：利用尿液样本的便利性和可用性，无创uADEV方法提供了一种新颖的工具，允许高频采样来调查快速发展的中枢神经系统疾病。

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来源期刊

International Journal of Nanomedicine NANOSCIENCE & NANOTECHNOLOGY-PHARMACOLOGY & PHARMACY

CiteScore

14.40

自引率

3.80%

发文量

511

审稿时长

1.4 months

期刊介绍： The International Journal of Nanomedicine is a globally recognized journal that focuses on the applications of nanotechnology in the biomedical field. It is a peer-reviewed and open-access publication that covers diverse aspects of this rapidly evolving research area. With its strong emphasis on the clinical potential of nanoparticles in disease diagnostics, prevention, and treatment, the journal aims to showcase cutting-edge research and development in the field. Starting from now, the International Journal of Nanomedicine will not accept meta-analyses for publication.