Macrolide sesquiterpene pyridine alkaloids from the roots of Tripterygium regelii and their anti-inflammatory activity

IF 4.5 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioorganic Chemistry Pub Date : 2025-03-01 DOI:10.1016/j.bioorg.2025.108330

Dawei Li , Qi Jia , Qi Zhao , Jintao Sun , Jianming Yu , Huan Chen , Linlin Sui , Aijing Leng , Ping Guo , Chao Wang

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Abstract

Eleven new macrolide sesquiterpene pyridine alkaloids (1–8 and 10–12) and twelve known congeners (9 and 13–23) were isolated from Tripterygium regelii roots. Their structures were identified through NMR, HRESIMS, and X-ray crystallography. Additionally, their anti-inflammatory activity was evaluated using a dual luciferase screening system based on aryl hydrocarbon receptor (AHR) activation, as well as a lipopolysaccharide (LPS)-induced macrophage model. Compounds 7 and 13 were found to significantly activate AHR, inhibit nitric oxide production, suppress the JNK and NF-κB/NLRP3 signaling pathways, and reduce inflammation-related proteins expression, including IL-6 and COX 2. This study not only expands the chemical variety of macrolide sesquiterpene pyridine alkaloids but also suggests that compounds 7 and 13 could be potential candidates for inflammation-related disease treatment.

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来源期刊

Bioorganic Chemistry 生物-生化与分子生物学

CiteScore

9.70

自引率

3.90%

发文量

679

审稿时长

31 days

期刊介绍： Bioorganic Chemistry publishes research that addresses biological questions at the molecular level, using organic chemistry and principles of physical organic chemistry. The scope of the journal covers a range of topics at the organic chemistry-biology interface, including: enzyme catalysis, biotransformation and enzyme inhibition; nucleic acids chemistry; medicinal chemistry; natural product chemistry, natural product synthesis and natural product biosynthesis; antimicrobial agents; lipid and peptide chemistry; biophysical chemistry; biological probes; bio-orthogonal chemistry and biomimetic chemistry. For manuscripts dealing with synthetic bioactive compounds, the Journal requires that the molecular target of the compounds described must be known, and must be demonstrated experimentally in the manuscript. For studies involving natural products, if the molecular target is unknown, some data beyond simple cell-based toxicity studies to provide insight into the mechanism of action is required. Studies supported by molecular docking are welcome, but must be supported by experimental data. The Journal does not consider manuscripts that are purely theoretical or computational in nature. The Journal publishes regular articles, short communications and reviews. Reviews are normally invited by Editors or Editorial Board members. Authors of unsolicited reviews should first contact an Editor or Editorial Board member to determine whether the proposed article is within the scope of the Journal.