Extraction, purification, and structural analysis of green pigments from Metarhizium flavoviride

IF 4.7 2区化学 Q2 CHEMISTRY, PHYSICAL Journal of Molecular Structure Pub Date : 2025-03-06 DOI:10.1016/j.molstruc.2025.141913

Jie Wei , Xue Zhao , Xiaoting Yang , Wei Jia , Jiangnan Qin , Wanxiang Li , Siying Li , Fenglin Hu , Yuhui Wang

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Abstract

Metarhizium exhibits a high tolerance to abiotic stress, which has led to its widespread use as a biopesticide in agricultural production. The green pigment in Metarhizium spores is important because it plays a key role in helping the spores resist abiotic stress. However, extracting this pigment with common solvents has proven difficult. This difficulty hinders both the identification and functional analysis of the pigment. In this study, we selected the high pigment-producing strain RCEF6989 of Metarhizium flavoviride for pigment extraction and identification. Extraction tests using acids, alkalis, enzymes, ionic reagents, and cell wall dissolution reagents revealed that formic acid is the most effective for extracting the green pigment. The green pigment was purified using preparative HPLC after extraction. The molecular formula of the pigment was determined by HPLC-PAD-ESI-HRMS to be C₁₈H₁₂N₈O₁₄. 1D and 2D NMR analyses revealed that the pigment is a novel compound, named metarhiziviridisin A. The identification of the pigment provides a scientific basis for further research into its function and biosynthetic pathways.

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黄绿绿僵菌中绿色色素的提取、纯化及结构分析

绿僵菌表现出对非生物胁迫的高度耐受性，这使得它作为生物农药在农业生产中被广泛使用。绿芽孢杆菌孢子中的绿色色素在帮助孢子抵抗非生物胁迫方面起着关键作用。然而，用普通溶剂提取这种色素已被证明是困难的。这一困难阻碍了色素的鉴定和功能分析。本研究选择黄绿绿僵菌高产色素菌株RCEF6989进行色素提取和鉴定。用酸、碱、酶、离子试剂和细胞壁溶解试剂进行的提取试验表明，甲酸是提取绿色色素最有效的方法。提取后采用制备高效液相色谱法纯化绿色色素。通过HPLC-PAD-ESI-HRMS测定该色素的分子式为C18H12N8O14。通过1D和2D NMR分析，发现该色素为一种新型化合物，命名为metarhiziviridisin a。该色素的鉴定为进一步研究其功能和生物合成途径提供了科学依据。

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来源期刊

Journal of Molecular Structure 化学-物理化学

CiteScore

7.10

自引率

15.80%

发文量

2384

审稿时长

45 days

期刊介绍： The Journal of Molecular Structure is dedicated to the publication of full-length articles and review papers, providing important new structural information on all types of chemical species including: • Stable and unstable molecules in all types of environments (vapour, molecular beam, liquid, solution, liquid crystal, solid state, matrix-isolated, surface-absorbed etc.) • Chemical intermediates • Molecules in excited states • Biological molecules • Polymers. The methods used may include any combination of spectroscopic and non-spectroscopic techniques, for example: • Infrared spectroscopy (mid, far, near) • Raman spectroscopy and non-linear Raman methods (CARS, etc.) • Electronic absorption spectroscopy • Optical rotatory dispersion and circular dichroism • Fluorescence and phosphorescence techniques • Electron spectroscopies (PES, XPS), EXAFS, etc. • Microwave spectroscopy • Electron diffraction • NMR and ESR spectroscopies • Mössbauer spectroscopy • X-ray crystallography • Charge Density Analyses • Computational Studies (supplementing experimental methods) We encourage publications combining theoretical and experimental approaches. The structural insights gained by the studies should be correlated with the properties, activity and/ or reactivity of the molecule under investigation and the relevance of this molecule and its implications should be discussed.