The Effect of a Polysaccharide from Helianthus tuberosus L. on the Antiproliferative Activity of the N-Glycoside Indolo[2,3-a]carbazole Derivative LCS-1269

Abstract

The antitumor activity of the N-glycoside derivative indolo[2,3-a]carbazole LCS-1269 with a polysaccharide from Helianthus tuberosus L. as an adjuvant agent was studied on a model of Lewis epidermoid lung carcinoma in mice of F1 hybrids (C₅₇Bl/₆ × DBA/₂). The antitumor effect was assessed by inhibition of tumor growth in treated animals in comparison with controls. As a result, it was found that the therapeutic effect of the combination was higher and longer than in monotherapy, with a decrease in tumor growth of Lewis lung carcinoma by 53–64% up to 28 days of follow-up. The use of the polysaccharide led to an increase in the number of blood cells, leukocytes, lymphocytes, and phagocytes responsible for antitumor immunity. The chemotherapeutic effect of the combination of LCS-1269 and polysaccharide showed a pronounced stable antitumor response against Lewis lung carcinoma in the peripheral blood system of mice with a temporary increase in the number of neutrophils and monocytes on the 12th day of tumor development. The tested compounds obviously stimulated the proliferation of certain phenotypes of neutrophils and monocytes with antitumor activity at an earlier stage of Lewis lung carcinoma development.