Chaihuang Qingyi Huoxue granule ameliorates severe acute pancreatitis by modulating gut microbiota and repairing the intestinal mucosal barrier.

IF 4.8 2区医学 Q2 IMMUNOLOGY Frontiers in Cellular and Infection Microbiology Pub Date : 2025-02-18 eCollection Date: 2025-01-01 DOI:10.3389/fcimb.2025.1514201

Xiaobin Zhang, Xusen Zeng, Wen Guo, Xin Zhou, Yi Zhang, Mingyun Tang, Juan Fu, Yuqing Deng, Xin Liang, Long Zhao, Zhi Li, Tiangang Wang, Li Li, Guohui Xiao

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Abstract

Background: During severe acute pancreatitis (SAP), damage to the intestinal mucosal barrier and translocation of intestinal pathogenic bacteria are key mechanisms that accelerate the disease progression of SAP. Chaihuang Qingyi Huoxue Granule (CH) is a herbal formula used in the clinical treatment of SAP. This study aims to investigate the role of CH in regulating gut microbiota and intestinal mucosal barrier in SAP rats.

Methods: Sodium taurocholate (3.5%) was retrogradely perfused into the biliopancreatic duct to establish the model of SAP in rats. CH (4.4 g/kg) was administered by gavage. Serum amylase, lipase, and endotoxin levels were measured. Hematoxylin-eosin (HE) staining was used to observe morphological changes in the pancreas and colon. The expression of zona occludens-1 (ZO-1) and occludin in the colon was examined by immunohistochemistry (IHC) and western blot. 16S rDNA gene sequencing was used to analyze the gut microbiota of the rats. The content of short-chain fatty acids (SCFAs) in the intestinal contents of the rats was determined by gas chromatography-mass spectrometry (GC-MS).

Results: CH reduced serum amylase, lipase, and endotoxin levels in SAP rats, alleviated pathological damage in the pancreas and colon, and restored the expression of ZO-1 and occludin. Moreover, CH alleviated gut microbiota dysbiosis in SAP rats, with restored gut microbiota diversity and structure. At the phylum level, the relative abundance of Firmicutes and Bacteroidetes increased, while that of Proteobacteria decreased. At the genus level, the abundance of Ruminococcus 1, Parabacteroides, Prevotellaceae UCG-001, Lachnospiraceae NK4A136 group, and Lactobacillus increased, while that of Escherichia-Shigella, Enterococcus, and Enterobacter decreased. In addition, CH increased the levels of SCFAs in the intestinal contents of SAP rats.

Conclusion: CH ameliorates SAP by maintaining the homeostasis and diversity of the gut microbiota, increasing the levels of SCFAs, and repairing the intestinal mucosal barrier.

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柴黄清益活血颗粒通过调节肠道菌群和修复肠黏膜屏障改善重症急性胰腺炎。

背景：在重症急性胰腺炎（SAP）发病过程中，肠黏膜屏障的损伤和肠道病原菌的易位是加速SAP病情进展的关键机制，柴黄清益活血颗粒（ch黄清益活血颗粒）是临床上治疗SAP的常用中药方剂，本研究旨在探讨ch黄清益活血颗粒在SAP大鼠肠道菌群和肠黏膜屏障的调节作用。方法：将3.5%牛磺胆酸钠逆行灌注胆管，建立大鼠SAP模型。灌胃给予CH （4.4 g/kg）。测定血清淀粉酶、脂肪酶和内毒素水平。苏木精-伊红（HE）染色观察胰腺和结肠的形态学变化。免疫组化（IHC）和western blot检测结肠组织中occludens-1 （ZO-1）和occludin的表达。采用16S rDNA基因测序法对大鼠肠道菌群进行分析。采用气相色谱-质谱联用技术测定大鼠肠道内容物中短链脂肪酸（SCFAs）的含量。结果：CH降低SAP大鼠血清淀粉酶、脂肪酶和内毒素水平，减轻胰腺和结肠病理损伤，恢复ZO-1和occludin的表达。此外，CH减轻了SAP大鼠肠道菌群失调，恢复了肠道菌群的多样性和结构。在门水平上，厚壁菌门和拟杆菌门的相对丰度增加，变形菌门的相对丰度降低。在属水平上，Ruminococcus 1、parabobacteroides、Prevotellaceae UCG-001、Lachnospiraceae NK4A136组和Lactobacillus的丰度增加，而Escherichia-Shigella、Enterococcus和Enterobacter的丰度降低。此外，CH增加了SAP大鼠肠道内容物中SCFAs的水平。结论：CH通过维持肠道微生物群的稳态和多样性、增加scfa水平和修复肠黏膜屏障来改善SAP。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Cellular and Infection Microbiology IMMUNOLOGY-MICROBIOLOGY

CiteScore

7.90

自引率

7.00%

发文量

1817

审稿时长

14 weeks

期刊介绍： Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.