{"title":"<i>Christiangramia sediminicola</i> sp. nov., a DNA-hydrolysing bacterium isolated from intertidal sediment.","authors":"Jia-Xuan Wang, Jing Wang, Ning-Hua Liu, Qi-Long Qin, Ping-Yi Li, Xiao-Yan Song, Xiu-Lan Chen, Yu-Zhong Zhang, Xi-Ying Zhang","doi":"10.1099/ijsem.0.006703","DOIUrl":null,"url":null,"abstract":"<p><p>A Gram-stain-negative, yellow-pigmented, non-flagellated, motile by gliding, aerobic and rod-shaped bacterial strain, designated SM2212<sup>T</sup>, was isolated from intertidal sediment of Aoshan Bay in Qingdao, China. The strain grew at 5-35 °C and with 0.5-5.5% NaCl (w/v). It was able to reduce nitrate to nitrite and hydrolyse starch, gelatin and DNA. The phylogenetic trees based on the 16S rRNA genes and single-copy genes showed that strain SM2212<sup>T</sup> belonged to the genus <i>Christiangramia</i> within the family <i>Flavobacteriaceae</i>, sharing the highest 16S rRNA gene sequence similarity with the type strain of <i>Christiangramia echinicola</i> (97.1%) and 96.2-97.0% 16S rRNA gene sequence similarity with those of other known species in the genus. The major cellular fatty acids were summed feature 3 (C<sub>16:1</sub> <i>ω7с</i> and/or C<sub>16:1</sub> <i>ω6с</i>), iso-C<sub>15:0</sub>, iso-C<sub>17: 0</sub>3-OH, summed feature 9 (iso-C<sub>17:1</sub> <i> ω9c</i> and/or 10-methyl C<sub>16:0</sub>) and C<sub>16:0</sub>. The major polar lipids were phosphatidylethanolamine and an unidentified lipid. The genomic DNA G+C content of strain SM2212<sup>T</sup> was 37.0 mol%. The digital DNA-DNA hybridization and average nucleotide identity values between strain SM2212<sup>T</sup> and type strains of closely related known <i>Christiangramia</i> species were below 22.2 and 79.7%, respectively. Based on the polyphasic taxonomic analysis in this study, strain SM2212<sup>T</sup> is considered to represent a novel species in the genus <i>Christiangramia</i>, for which the name <i>Christiangramia sediminicola</i> is proposed. The type strain is SM2212<sup>T</sup> (=KCTC 92980<sup>T</sup>=MCCC 1K07684<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of systematic and evolutionary microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006703","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
A Gram-stain-negative, yellow-pigmented, non-flagellated, motile by gliding, aerobic and rod-shaped bacterial strain, designated SM2212T, was isolated from intertidal sediment of Aoshan Bay in Qingdao, China. The strain grew at 5-35 °C and with 0.5-5.5% NaCl (w/v). It was able to reduce nitrate to nitrite and hydrolyse starch, gelatin and DNA. The phylogenetic trees based on the 16S rRNA genes and single-copy genes showed that strain SM2212T belonged to the genus Christiangramia within the family Flavobacteriaceae, sharing the highest 16S rRNA gene sequence similarity with the type strain of Christiangramia echinicola (97.1%) and 96.2-97.0% 16S rRNA gene sequence similarity with those of other known species in the genus. The major cellular fatty acids were summed feature 3 (C16:1ω7с and/or C16:1ω6с), iso-C15:0, iso-C17: 03-OH, summed feature 9 (iso-C17:1 ω9c and/or 10-methyl C16:0) and C16:0. The major polar lipids were phosphatidylethanolamine and an unidentified lipid. The genomic DNA G+C content of strain SM2212T was 37.0 mol%. The digital DNA-DNA hybridization and average nucleotide identity values between strain SM2212T and type strains of closely related known Christiangramia species were below 22.2 and 79.7%, respectively. Based on the polyphasic taxonomic analysis in this study, strain SM2212T is considered to represent a novel species in the genus Christiangramia, for which the name Christiangramia sediminicola is proposed. The type strain is SM2212T (=KCTC 92980T=MCCC 1K07684T).
期刊介绍:
Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names.
The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including:
Identification, characterisation and culture preservation
Microbial evolution and biodiversity
Molecular environmental work with strong taxonomic or evolutionary content
Nomenclature
Taxonomy and phylogenetics.