Identification of reproductive performance in Bali-polled bulls using computer-assisted semen analysis and plasma seminal proteomics.

IF 1.7 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Veterinary World Pub Date : 2025-01-01 Epub Date: 2025-01-14 DOI:10.14202/vetworld.2025.102-109

Athhar Manabi Diansyah, Sanstoso Santoso, Herdis Herdis, Muhammad Yusuf, Tri Puji Priyatno, Tulus Maulana, Abdul Latief Toleng, Muhammad Ihsan Andi Dagong, Syahruddin Said, Hikmayani Iskandar, Aeni Nurlatifah, Puji Lestari, Lukman Affandy, Abdullah Baharun

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引用次数: 0

Abstract

Background and aim: Bali-polled bulls, known for their favorable traits, require advanced reproductive performance analysis to optimize breeding programs. This study aimed to evaluate sperm kinematics and seminal plasma proteomic profiles as biomarkers for sperm motility and fertility in Bali-polled bulls.

Materials and methods: Semen from five Bali-polled bulls was collected biweekly over five batches using artificial vaginas. Sperm kinematics were assessed using computer-assisted semen analysis (CASA). Fertility was evaluated through service per conception (S/C) in artificial insemination trials. Seminal plasma proteins were analyzed through liquid chromatography-tandem mass spectrometry (LC-MS/MS) and annotated using the UniProt database, PANTHER for gene ontology, and STRING database for protein interactions.

Results: Post-thaw sperm kinematics showed satisfactory results, with a mean S/C of 1.52. Proteomic analysis identified 138 proteins, including six (TEX101, BSP1, PRSS55, BSP3, SPADH2, and TPPP2) linked to sperm motility. These proteins were involved in key biological processes such as sperm capacitation, motility regulation, and sperm-oocyte interaction.

Conclusion: Sperm kinematics and seminal plasma proteomics provide insights into Bali-polled bull fertility. Identified proteins can serve as fertility biomarkers, aiding in superior local breed development and reproductive efficiency improvement.

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来源期刊

Veterinary World Multiple-

CiteScore

3.60

自引率

12.50%

发文量

317

审稿时长

16 weeks

期刊介绍： Veterinary World publishes high quality papers focusing on Veterinary and Animal Science. The fields of study are bacteriology, parasitology, pathology, virology, immunology, mycology, public health, biotechnology, meat science, fish diseases, nutrition, gynecology, genetics, wildlife, laboratory animals, animal models of human infections, prion diseases and epidemiology. Studies on zoonotic and emerging infections are highly appreciated. Review articles are highly appreciated. All articles published by Veterinary World are made freely and permanently accessible online. All articles to Veterinary World are posted online immediately as they are ready for publication.