Identification of reproductive performance in Bali-polled bulls using computer-assisted semen analysis and plasma seminal proteomics.

IF 2 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Veterinary World Pub Date : 2025-01-01 Epub Date: 2025-01-14 DOI:10.14202/vetworld.2025.102-109

Athhar Manabi Diansyah, Sanstoso Santoso, Herdis Herdis, Muhammad Yusuf, Tri Puji Priyatno, Tulus Maulana, Abdul Latief Toleng, Muhammad Ihsan Andi Dagong, Syahruddin Said, Hikmayani Iskandar, Aeni Nurlatifah, Puji Lestari, Lukman Affandy, Abdullah Baharun

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Abstract

Background and aim: Bali-polled bulls, known for their favorable traits, require advanced reproductive performance analysis to optimize breeding programs. This study aimed to evaluate sperm kinematics and seminal plasma proteomic profiles as biomarkers for sperm motility and fertility in Bali-polled bulls.

Materials and methods: Semen from five Bali-polled bulls was collected biweekly over five batches using artificial vaginas. Sperm kinematics were assessed using computer-assisted semen analysis (CASA). Fertility was evaluated through service per conception (S/C) in artificial insemination trials. Seminal plasma proteins were analyzed through liquid chromatography-tandem mass spectrometry (LC-MS/MS) and annotated using the UniProt database, PANTHER for gene ontology, and STRING database for protein interactions.

Results: Post-thaw sperm kinematics showed satisfactory results, with a mean S/C of 1.52. Proteomic analysis identified 138 proteins, including six (TEX101, BSP1, PRSS55, BSP3, SPADH2, and TPPP2) linked to sperm motility. These proteins were involved in key biological processes such as sperm capacitation, motility regulation, and sperm-oocyte interaction.

Conclusion: Sperm kinematics and seminal plasma proteomics provide insights into Bali-polled bull fertility. Identified proteins can serve as fertility biomarkers, aiding in superior local breed development and reproductive efficiency improvement.

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利用计算机辅助精液分析和血浆精液蛋白质组学鉴定巴厘调查公牛的生殖性能。

背景与目的：以优良性状著称的巴厘杂交公牛需要先进的繁殖性能分析来优化育种计划。本研究旨在评估精子运动学和精浆蛋白质组学特征作为巴厘岛调查公牛精子活力和生育能力的生物标志物。材料与方法：采用人工阴道，每两周采集5头巴厘公牛的精液。使用计算机辅助精液分析（CASA）评估精子运动学。在人工授精试验中，通过单次受孕服务（S/C）来评价生育能力。精浆蛋白通过液相色谱-串联质谱（LC-MS/MS）分析，并使用UniProt数据库、PANTHER基因本体数据库和STRING蛋白质相互作用数据库进行注释。结果：解冻后精子运动学结果令人满意，平均S/C为1.52。蛋白质组学分析鉴定出138个蛋白，包括6个与精子活力相关的蛋白（TEX101、BSP1、PRSS55、BSP3、SPADH2和TPPP2）。这些蛋白参与了关键的生物学过程，如精子获能、运动调节和精子-卵细胞相互作用。结论：精子运动学和精浆蛋白质组学为研究巴厘公牛的生育能力提供了新的思路。鉴定出的蛋白质可作为育性生物标记物，帮助本地优良品种的发育和繁殖效率的提高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary World Multiple-

CiteScore

3.60

自引率

12.50%

发文量

317

审稿时长

16 weeks

期刊介绍： Veterinary World publishes high quality papers focusing on Veterinary and Animal Science. The fields of study are bacteriology, parasitology, pathology, virology, immunology, mycology, public health, biotechnology, meat science, fish diseases, nutrition, gynecology, genetics, wildlife, laboratory animals, animal models of human infections, prion diseases and epidemiology. Studies on zoonotic and emerging infections are highly appreciated. Review articles are highly appreciated. All articles published by Veterinary World are made freely and permanently accessible online. All articles to Veterinary World are posted online immediately as they are ready for publication.