Limited Risk of Microbial Contamination During Hypothermic and Normothermic Machine Perfusion of Donor Kidneys.

IF 2.6 4区医学 Q3 IMMUNOLOGY Transplant Infectious Disease Pub Date : 2025-05-01 Epub Date: 2025-03-06 DOI:10.1111/tid.70019

Julia S Slagter, Carolina A M Schurink, Ga-Lai M Chong, Marlies E J Reinders, Robert J Porte, Robert C Minnee

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Abstract

Background: Both hypothermic machine perfusion (HMP) and normothermic machine perfusion (NMP) are increasingly used for preservation of deceased donor kidneys. However, especially NMP can pose as a risk for microbial contamination of the kidney graft as the 37°C perfusate can act as a breeding ground for microbial contaminants.

Methods: In this study, we investigated the cultures of HMP and NMP perfusates of deceased donor kidneys.

Results: Between January 2021 and April 2024, a total of 99 perfusates were examined (73 HMP and 26 NMP perfusates)-. We found that contamination of HMP perfusate was common, occurring in 21 of 73 cultures (29%). Most bacteria originated from the skin. Microbial contamination during NMP was rare, occurring only in 2 of 26 cultures (8%). Microbial contamination during machine perfusion did not lead to infectious complications in the recipients.

Conclusion: Machine perfusion poses a very limited risk of infection in kidney transplant recipients.

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供肾低温和常温机器灌注过程中微生物污染的有限风险。

背景：低温机器灌注（HMP）和常温机器灌注（NMP）越来越多地用于保存已故供体肾脏。然而，特别是NMP可能会对移植肾造成微生物污染的风险，因为37°C的灌注液可能成为微生物污染物的滋生地。方法：在本研究中，我们研究了死亡供肾HMP和NMP灌注液的培养。结果：在2021年1月至2024年4月期间，共检查了99例灌注器（HMP 73例，NMP 26例）。我们发现HMP灌注物污染很常见，在73个培养物中有21个（29%）发生。大多数细菌来自皮肤。NMP过程中微生物污染很少见，26个培养中只有2个（8%）发生。机器灌注过程中的微生物污染未导致受者感染并发症。结论：机器灌注对肾移植受者的感染风险非常有限。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transplant Infectious Disease 医学-传染病学

CiteScore

5.30

自引率

7.70%

发文量

210

审稿时长

4-8 weeks

期刊介绍： Transplant Infectious Disease has been established as a forum for presenting the most current information on the prevention and treatment of infection complicating organ and bone marrow transplantation. The point of view of the journal is that infection and allograft rejection (or graft-versus-host disease) are closely intertwined, and that advances in one area will have immediate consequences on the other. The interaction of the transplant recipient with potential microbial invaders, the impact of immunosuppressive strategies on this interaction, and the effects of cytokines, growth factors, and chemokines liberated during the course of infections, rejection, or graft-versus-host disease are central to the interests and mission of this journal. Transplant Infectious Disease is aimed at disseminating the latest information relevant to the infectious disease complications of transplantation to clinicians and scientists involved in bone marrow, kidney, liver, heart, lung, intestinal, and pancreatic transplantation. The infectious disease consequences and concerns regarding innovative transplant strategies, from novel immunosuppressive agents to xenotransplantation, are very much a concern of this journal. In addition, this journal feels a particular responsibility to inform primary care practitioners in the community, who increasingly are sharing the responsibility for the care of these patients, of the special considerations regarding the prevention and treatment of infection in transplant recipients. As exemplified by the international editorial board, articles are sought throughout the world that address both general issues and those of a more restricted geographic import.