Computer-Assisted Modeling and Simulation of a Dielectrophoresis-based Microseparator for Blood Cells Separation Applications

IF 1.3 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS Chromatographia Pub Date : 2025-02-05 DOI:10.1007/s10337-025-04385-9

Elnaz Poorreza

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Abstract

The objective of the present numerical investigation is to propose a microseparator specifically engineered for the separation of platelets from white blood cells (WBCs). This microfluidic device, which incorporates circular electrodes operating at a minimal voltage of 1.4 V, and a frequency of 100 kHz, is recommended for the targeted separation of platelets and WBCs utilizing dielectrophoresis (DEP) force. The utilization of a low-voltage level ensures the preservation of the viability of biological cells, a fundamental consideration in medical applications. Simulation results are presented to illustrate the electric potential, electric field, velocity, pressure, and DEP force profiles concerning two distinct particles. Through a comparative analysis employing the finite element method, we delineate the implications of modulating the inlet velocity field on the pressure exerted upon the particles. Subsequently, the impact of varying the fluid conductivity and input voltage on electrodes within the microchannel on particle separation was examined. It is anticipated that this comprehensive design is exceptionally conducive to the realization of DEP-based practical biochips for cell separation.

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基于介电泳的血细胞分离微分离器的计算机辅助建模与仿真

本数值研究的目的是提出一种专门用于从白细胞（wbc）中分离血小板的微分离器。这种微流控装置，包括在1.4 V的最小电压下工作的圆形电极，频率为100 kHz，被推荐用于利用介电电泳（DEP）力靶向分离血小板和白细胞。低电压水平的利用确保了生物细胞活力的保存，这是医疗应用中的一个基本考虑因素。模拟结果说明了两个不同粒子的电势、电场、速度、压力和DEP力分布。通过采用有限元方法的对比分析，我们描述了调节入口速度场对施加在颗粒上的压力的影响。随后，研究了改变微通道内电极上流体电导率和输入电压对颗粒分离的影响。预计这一综合设计将特别有利于实现基于dep的细胞分离实用生物芯片。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.