HCC-derived CX3CL1 affects hepatocellular carcinoma prognosis and CX3CR1 + MDSC infiltration.

IF 3.4 3区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL European Journal of Medical Research Pub Date : 2025-03-06 DOI:10.1186/s40001-025-02410-z

Xiaoling Zhang, Yidan Lou, Song Zheng, Xu Chang

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Abstract

Background: Hepatocellular carcinoma (HCC) remains a leading cause of cancer-related mortality worldwide, largely because of its ability to reshape the tumor microenvironment and evade immune surveillance.

Methods: CX3CL1 expression in HCC tumor tissues was assessed via immunohistochemistry, while plasma levels were quantified using enzyme-linked immunosorbent assays (ELISAs). CX3CR1-positive immune cell infiltration was analyzed by immunofluorescence. The associations among CX3CL1 expression, CX3CR1-positive cell infiltration, and patient prognosis were examined. Additionally, cell-based assays were conducted to evaluate the impact of CX3CL1 amplification on the expression of myeloid-derived suppressor cell (MDSC)-recruiting factors.

Results: Elevated CX3CL1 levels were significantly correlated with increased MDSC infiltration in the tumor microenvironment and poorer patient prognosis. CX3CL1 amplification led to the upregulation of MDSC-recruiting factors, suggesting a potential mechanism for immune evasion.

Conclusions: These findings highlight the possible involvement of CX3CL1 in HCC progression via MDSC recruitment, suggesting that it is a promising therapeutic target for promoting antitumor immunity. Further studies are needed to confirm these findings and explore potential therapeutic strategies.

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hcc来源的CX3CL1影响肝癌预后和CX3CR1 + MDSC浸润。

背景：肝细胞癌（HCC）仍然是世界范围内癌症相关死亡的主要原因，主要是因为其重塑肿瘤微环境和逃避免疫监视的能力。方法：采用免疫组化法检测CX3CL1在HCC肿瘤组织中的表达，采用酶联免疫吸附法（elisa）检测血浆中CX3CL1的表达水平。免疫荧光法分析cx3cr1阳性免疫细胞浸润情况。观察CX3CL1表达、cx3cr1阳性细胞浸润与患者预后的关系。此外，我们还进行了基于细胞的检测，以评估CX3CL1扩增对髓源性抑制细胞（MDSC）招募因子表达的影响。结果：CX3CL1水平升高与肿瘤微环境中MDSC浸润增加及患者预后不良显著相关。CX3CL1扩增导致mdsc招募因子上调，提示免疫逃避的潜在机制。结论：这些发现强调了CX3CL1可能通过MDSC募集参与HCC的进展，表明它是促进抗肿瘤免疫的有希望的治疗靶点。需要进一步的研究来证实这些发现并探索潜在的治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Journal of Medical Research 医学-医学：研究与实验

CiteScore

3.20

自引率

0.00%

发文量

247

审稿时长

>12 weeks

期刊介绍： European Journal of Medical Research publishes translational and clinical research of international interest across all medical disciplines, enabling clinicians and other researchers to learn about developments and innovations within these disciplines and across the boundaries between disciplines. The journal publishes high quality research and reviews and aims to ensure that the results of all well-conducted research are published, regardless of their outcome.