Twenty thousand fishes under the seas: Insights into the collection and storage of trematodes from the examination of 20,000 fishes in the tropical Indo west-Pacific.

Abstract

The techniques employed to collect and store trematodes vary between research groups, and although these differences are sometimes necessitated by distinctions in the hosts examined, they are more commonly an artefact of instruction. As a general rule, we tend to follow what we were taught rather than explore new techniques. A major reason for this is that there are few technique papers in the published literature. Inspired by a collaborative workshop at the Trematodes 2024 symposium, we outline our techniques and processes for collecting adult trematodes from fishes and discuss the improvements we have made over 40 years of dissections of 20,000+ individual marine fishes. We present these techniques for two reasons: first, to encourage unified methods across the globe, with an aim to produce optimally comparable specimens across temporal periods, across geographic localities, and between research groups; and second, as a resource for inexperienced researchers. We stress the importance of understanding differences in host biology and the expected trematode fauna, which ultimately enables organised and productive dissections. We outline our dissection method for each key organ separately, discuss handling, fixation, and storage methods to generate the most uniform and comparable samples, and explore ethical considerations, issues of accurate host identification, and the importance and potential of clear record keeping.