LncRNA SNHG14 Delivered by Bone Marrow Mesenchymal Stem Cells-Secreted Exosomes Regulates Osteogenesis and Adipogenesis in Osteoporosis by Mediating the miR-27a-3p/LMNB1 Axis.

Abstract

The purpose of this study was to investigate the role of LncRNA SNHG14 delivered by bone marrow mesenchymal stem cells-secreted exosomes (BMSC-Exos) in osteoporosis (OP). BMSCs and BMSCs-Exos were isolated and identified. BMSCs were transfected, from which BMSCs-Exos were collected. The treated BMSCs-Exos were co-cultured with BMSCs. After osteogenic induction of BMSCs, the calcification was analyzed by alizarin red S staining. After adipogenic induction of BMSCs, lipid droplets were detected by oil red O staining. Glycerol-3-phosphate dehydrogenase activity was measured in BMSCs. OVX mouse models were established and treated with BMSC-EXOs. HE staining and IHC staining were performed on the femurs of mice. The interaction between SNHG14, miR-27a-3p, and LMNB1 was evaluated by luciferase reporter gene assay and RIP assay. Gene levels were assessed using RT-qPCR and Western blot, respectively. BMSC-Exos promoted osteogenic-adipogenic balance of BMSCs. SNHG14 enhanced the promoting effect of BMSCs-Exos on the balance of osteogenesis and adipogenesis of BMSCs. SNHG14 directly bound miR-27a-3p. SNHG1 mediated osteogenic-adipogenic balance in BMSCs via miR-27a-3p. LMNB1 was a target gene of miR-27a-3p. LMNB1 was involved in the process of SNHG14 regulating osteogenic-adipogenic balance in BMSCs. SNHG14 overexpression promoted bone formation and alleviated OP in vivo. SNHG14 delivered by BMSCs-Exos regulates osteogenesis and adipogenesis in OP by mediating the miR-27a-3p/LMNB1 axis.