Light-Blocking Nanofiber Membranes Facilitating Physiologically Relevant In Situ Transmigration Assay

IF 5.4 2区 医学 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Biomaterials Science & Engineering Pub Date : 2025-02-20 DOI:10.1021/acsbiomaterials.4c0209610.1021/acsbiomaterials.4c02096
Dohui Kim, Soojin Yi, Byeong-ung Park, Seongsu Eom, Sinsung Kang, Dong Sung Kim* and Hong Kyun Kim*, 
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Abstract

Nanofiber (NF) membranes have demonstrated considerable potential in cellular transmigration studies due to their resemblance to the biophysical properties of basement membranes, enabling cellular behaviors that closely mimic those observed in vivo. Despite their advantages, conventional NF membranes often encounter issues in transmigration assays due to their transparency, which leads to overlapping fluorescent signals from transmigrated and nontransmigrated cells. This overlap complicates the clear differentiation between these cell populations, making the quantitative evaluation of live-cell transmigration challenging. To address this issue, we developed a light-blocking nanofiber (LB-NF) membrane by incorporating carbon black into polycaprolactone NFs. This LB-NF membrane is designed not only to mimic the biophysical properties of the basement membrane but also to enable in situ analysis of transmigrated cells through its light-blocking properties. Our study demonstrated the effectiveness of the LB-NF membrane in a transmigration assay using human brain cerebral microvascular endothelial cells (HBEC-5i), enabling physiologically relevant cell transmigration while significantly enhancing the accuracy of in situ fluorescence detection. Furthermore, drug testing within a choroidal neovascularization model using the LB-NF membrane underscores its utility and potential impact on pharmaceutical development, particularly for diseases involving abnormal cell transmigration. Therefore, the developed LB-NF membrane represents a valuable tool for the precise assessment of in situ cellular transmigration and holds significant promise for advancing drug screening and therapeutic development.

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纳米纤维(NF)膜与基底膜的生物物理特性相似,能使细胞行为与体内观察到的细胞行为非常相似,因此在细胞迁移研究中具有相当大的潜力。尽管传统的 NF 膜有很多优点,但由于其透明度问题,它们在转运试验中经常遇到问题,导致转运细胞和非转运细胞的荧光信号重叠。这种重叠使明确区分这些细胞群变得复杂,从而使活体细胞转运的定量评估变得具有挑战性。为了解决这个问题,我们在聚己内酯纳米纤维中加入了炭黑,开发出了一种阻光纳米纤维(LB-NF)膜。这种 LB-NF 膜不仅能模拟基底膜的生物物理特性,还能通过其阻光特性对迁移细胞进行原位分析。我们的研究证明了 LB-NF 膜在使用人脑微血管内皮细胞(HBEC-5i)进行转运试验中的有效性,在实现生理学相关细胞转运的同时,显著提高了原位荧光检测的准确性。此外,利用 LB-NF 膜在脉络膜新生血管模型中进行的药物测试也凸显了它的实用性和对药物开发的潜在影响,尤其是针对涉及异常细胞迁移的疾病。因此,所开发的 LB-NF 膜是精确评估原位细胞迁移的宝贵工具,在推进药物筛选和治疗开发方面具有重大前景。
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来源期刊
ACS Biomaterials Science & Engineering
ACS Biomaterials Science & Engineering Materials Science-Biomaterials
CiteScore
10.30
自引率
3.40%
发文量
413
期刊介绍: ACS Biomaterials Science & Engineering is the leading journal in the field of biomaterials, serving as an international forum for publishing cutting-edge research and innovative ideas on a broad range of topics: Applications and Health – implantable tissues and devices, prosthesis, health risks, toxicology Bio-interactions and Bio-compatibility – material-biology interactions, chemical/morphological/structural communication, mechanobiology, signaling and biological responses, immuno-engineering, calcification, coatings, corrosion and degradation of biomaterials and devices, biophysical regulation of cell functions Characterization, Synthesis, and Modification – new biomaterials, bioinspired and biomimetic approaches to biomaterials, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, genetic biomaterials design, synthetic biology, new composite systems, bionics, polymer synthesis Controlled Release and Delivery Systems – biomaterial-based drug and gene delivery, bio-responsive delivery of regulatory molecules, pharmaceutical engineering Healthcare Advances – clinical translation, regulatory issues, patient safety, emerging trends Imaging and Diagnostics – imaging agents and probes, theranostics, biosensors, monitoring Manufacturing and Technology – 3D printing, inks, organ-on-a-chip, bioreactor/perfusion systems, microdevices, BioMEMS, optics and electronics interfaces with biomaterials, systems integration Modeling and Informatics Tools – scaling methods to guide biomaterial design, predictive algorithms for structure-function, biomechanics, integrating bioinformatics with biomaterials discovery, metabolomics in the context of biomaterials Tissue Engineering and Regenerative Medicine – basic and applied studies, cell therapies, scaffolds, vascularization, bioartificial organs, transplantation and functionality, cellular agriculture
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