Disruption of the endoplasmic reticulum-localized fatty acyl-ACP thioesterase IPF1 caused partial male sterility in rice.

IF 3.8 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Plant Molecular Biology Pub Date : 2025-03-08 DOI:10.1007/s11103-025-01574-w

Wenye Tan, Jingfei Tian, Wenfeng Zhao, Jianxin Wei, Yibo Xu, Shixu Zhou, Zihan Wei, Zejun Shen, Minghang Wu, Lianguang Shang, Rongbai Li, Yongfei Wang, Baoxiang Qin

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Abstract

The fatty acyl ACP thioesterases, catalyzing the final step of fatty acid synthesis in the plastid, regulate various critical processes in plants, including seed oil accumulation, seed development, plant growth, and drought tolerance. However, their roles in male fertility have seldom been demonstrated. In this study, the function of a newly FAT, Impaired Pollen Fertility 1 (IPF1) in male fertility was investigated. IPF1 expressed prominently in microspores and tapetum. IPF1 specifically located in the endoplasmic reticulum. IPF1 knock-out mutants produced by the CRISPR/Cas9 system displayed significant reduction in seed-setting rate compared to WT. The decreased seed-setting rate in the ipf1 mutants was found to be attributed to the defects of pollen viability, not the female gamete fertility. The aborted pollen in the ipf1 mutants showed impaired pollen wall formation and diminished lipid deposition. Consistently, the expression levels of six genes critical to pollen wall formation and lipid metabolism (GPAT3, OsC6, DPW2, OsPKS1, OsPKS2, and OsSTRL2) were significantly decreased in the ipf1 mutant. Taken together, these results demonstrate that IPF1 regulates rice pollen fertility through the modulation of lipid synthesis.

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内质网脂肪酰基- acp硫酯酶IPF1的破坏导致水稻部分雄性不育。

脂肪酸酰基ACP硫酯酶是催化质体中脂肪酸合成的最后一步，调控植物的多种关键过程，包括种子油脂积累、种子发育、植物生长和耐旱性。然而，它们在男性生育能力中的作用很少得到证实。本研究研究了花粉育性受损1 （IPF1）在雄性植物育性中的作用。IPF1在小孢子和绒毡层中显著表达。IPF1特异性位于内质网。与WT相比，CRISPR/Cas9系统产生的IPF1敲除突变体的结实率显著降低。发现IPF1突变体的结实率下降是由于花粉活力缺陷，而不是由于雌性配子的育性缺陷。ipf1突变体的败育花粉表现为花粉壁形成受损和脂质沉积减少。在ipf1突变体中，与花粉壁形成和脂质代谢有关的6个关键基因（GPAT3、OsC6、DPW2、OsPKS1、OsPKS2和OsSTRL2）的表达水平也显著降低。综上所述，这些结果表明IPF1通过调节脂质合成来调节水稻花粉的育性。

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来源期刊

Plant Molecular Biology 生物-生化与分子生物学

自引率

2.00%

发文量

审稿时长

1.4 months

期刊介绍： Plant Molecular Biology is an international journal dedicated to rapid publication of original research articles in all areas of plant biology.The Editorial Board welcomes full-length manuscripts that address important biological problems of broad interest, including research in comparative genomics, functional genomics, proteomics, bioinformatics, computational biology, biochemical and regulatory networks, and biotechnology. Because space in the journal is limited, however, preference is given to publication of results that provide significant new insights into biological problems and that advance the understanding of structure, function, mechanisms, or regulation. Authors must ensure that results are of high quality and that manuscripts are written for a broad plant science audience.