Feifei Ma , Jiameng Guo , Yantao Li , Gao Li , Xuezhi Zhang , Zhuoyi Zhu , Roger Ruan , Pengfei Cheng
{"title":"Optimizing Fucoxanthin production in Chaetoceros sp. Using conditioned wastewater and tailored culture conditions","authors":"Feifei Ma , Jiameng Guo , Yantao Li , Gao Li , Xuezhi Zhang , Zhuoyi Zhu , Roger Ruan , Pengfei Cheng","doi":"10.1016/j.jwpe.2025.107450","DOIUrl":null,"url":null,"abstract":"<div><div>Fucoxanthin is a naturally occurring carotenoid with various biological activities. Compared to macroalgae, microalgae (such as diatoms) not only have a faster growth rate but also can accumulate higher levels of fucoxanthin. However, the cost of the culture medium is a crucial limiting factor in the large-scale production of microalgal fucoxanthin. In this study, it was first screened for microalgal strains with optimal fucoxanthin production based on aquaculture wastewater. System factors such as initial inoculation density, nitrogen‑phosphorus (N/P) ratio, and light intensity were then optimized. Further, pilot-scale cultivation of <em>Chaetoceros</em> sp. was conducted based on the optimized conditions, using response surface methodology. These results showed that the fucoxanthin production for <em>Chaetoceros</em> sp. ZJ was best when the ratio of aquaculture wastewater to culture medium was 1:3. Optimal inoculation density was 2.6 × 10<sup>5</sup> cells/mL; the nitrogen‑phosphorus ratio was 25.61 to 1.0; the light intensity was 1597.74 lx.; the resulting yield of fucoxanthin reached 40.13 mg/L under these conditions. In a 100-Liter tubular photobioreactor, the yield of fucoxanthin for <em>Chaetoceros</em> sp. ZJ reached 33 mg/L. This study provides a theoretical basis for the utilization of wastewater to cultivate <em>Chaetoceros</em> sp., and to create fucoxanthin as a commercial resource. Using nutrient-rich wastewater for microalgae cultivation enables both environmental pollution mitigation and valuable bioproduct generation.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"72 ","pages":"Article 107450"},"PeriodicalIF":6.3000,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of water process engineering","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214714425005227","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Fucoxanthin is a naturally occurring carotenoid with various biological activities. Compared to macroalgae, microalgae (such as diatoms) not only have a faster growth rate but also can accumulate higher levels of fucoxanthin. However, the cost of the culture medium is a crucial limiting factor in the large-scale production of microalgal fucoxanthin. In this study, it was first screened for microalgal strains with optimal fucoxanthin production based on aquaculture wastewater. System factors such as initial inoculation density, nitrogen‑phosphorus (N/P) ratio, and light intensity were then optimized. Further, pilot-scale cultivation of Chaetoceros sp. was conducted based on the optimized conditions, using response surface methodology. These results showed that the fucoxanthin production for Chaetoceros sp. ZJ was best when the ratio of aquaculture wastewater to culture medium was 1:3. Optimal inoculation density was 2.6 × 105 cells/mL; the nitrogen‑phosphorus ratio was 25.61 to 1.0; the light intensity was 1597.74 lx.; the resulting yield of fucoxanthin reached 40.13 mg/L under these conditions. In a 100-Liter tubular photobioreactor, the yield of fucoxanthin for Chaetoceros sp. ZJ reached 33 mg/L. This study provides a theoretical basis for the utilization of wastewater to cultivate Chaetoceros sp., and to create fucoxanthin as a commercial resource. Using nutrient-rich wastewater for microalgae cultivation enables both environmental pollution mitigation and valuable bioproduct generation.
期刊介绍:
The Journal of Water Process Engineering aims to publish refereed, high-quality research papers with significant novelty and impact in all areas of the engineering of water and wastewater processing . Papers on advanced and novel treatment processes and technologies are particularly welcome. The Journal considers papers in areas such as nanotechnology and biotechnology applications in water, novel oxidation and separation processes, membrane processes (except those for desalination) , catalytic processes for the removal of water contaminants, sustainable processes, water reuse and recycling, water use and wastewater minimization, integrated/hybrid technology, process modeling of water treatment and novel treatment processes. Submissions on the subject of adsorbents, including standard measurements of adsorption kinetics and equilibrium will only be considered if there is a genuine case for novelty and contribution, for example highly novel, sustainable adsorbents and their use: papers on activated carbon-type materials derived from natural matter, or surfactant-modified clays and related minerals, would not fulfil this criterion. The Journal particularly welcomes contributions involving environmentally, economically and socially sustainable technology for water treatment, including those which are energy-efficient, with minimal or no chemical consumption, and capable of water recycling and reuse that minimizes the direct disposal of wastewater to the aquatic environment. Papers that describe novel ideas for solving issues related to water quality and availability are also welcome, as are those that show the transfer of techniques from other disciplines. The Journal will consider papers dealing with processes for various water matrices including drinking water (except desalination), domestic, urban and industrial wastewaters, in addition to their residues. It is expected that the journal will be of particular relevance to chemical and process engineers working in the field. The Journal welcomes Full Text papers, Short Communications, State-of-the-Art Reviews and Letters to Editors and Case Studies