A Freely Moving Photothrombotic Stroke Model Reveals Sustained Dysfunction of GABAergic Neuron in Contralesional Cortex Using Miniaturized Two-Photon Microscopy.
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引用次数: 0
Abstract
Ischemic stroke (IS) commonly results in long-term disability, largely due to alterations in neuronal networks. In repeatable rodent IS model under naturalistic conditions, the difficulty of capturing single-cell neuronal activities and how this solves a long-standing challenge is still remained. Here, we combined a photothrombotic stroke model with head-mounted miniaturized two-photon microscopy (mTPM) to achieve longitudinal, real-time imaging of GABAergic neurons in the contralesional primary motor cortex (M1) in freely moving mice. We observed pronounced reductions in calcium dynamics in GABAergic neurons. These calcium dynamics emerged as early as day 3 post-stroke and persisted through day 19, despite no detectable gross motor deficits. Our findings highlight subtle cortical dysfunction persists despite normal gross motor function, underscoring the need for finer behavioral tests. This approach offered a powerful tool to bridge the gap between cellular-level dysfunction and macroscopic behaviors after focal ischemic stroke.
期刊介绍:
Translational Stroke Research covers basic, translational, and clinical studies. The Journal emphasizes novel approaches to help both to understand clinical phenomenon through basic science tools, and to translate basic science discoveries into the development of new strategies for the prevention, assessment, treatment, and enhancement of central nervous system repair after stroke and other forms of neurotrauma.
Translational Stroke Research focuses on translational research and is relevant to both basic scientists and physicians, including but not restricted to neuroscientists, vascular biologists, neurologists, neuroimagers, and neurosurgeons.