Expression profile and N6-methyadenosine modification of circular RNA analysis in MAFLD.

IF 2.5 3区医学 Q2 GASTROENTEROLOGY & HEPATOLOGY BMC Gastroenterology Pub Date : 2025-03-11 DOI:10.1186/s12876-025-03722-4

Mengyao Zheng, Dongyun Cun, Haiyu He, Xuancheng Xie, Hongtao Lei, Wen Fu, Wenlin Tai, Jinhui Yang

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引用次数: 0

Abstract

Background: To analyze the expression patterns of circRNAs in metabolic associated fatty liver disease (MAFLD) and the regulation of m⁶A methylation on those circRNAs.

Methods: The expression profile of CircRNA in MAFLD and normal control liver tissues was analyzed by microarray. Predict the potential m⁶A sites of the differentially expression circRNAs (DECs) via the SRAMP website. The biological functions and molecular interactions of DECs were analyzed by GO and KEGG analyses. The selected DECs were verified by MeRIP-qPCR and RT-qPCR.

Results: There were 59 DECs in MAFLD liver tissues compared with normal control liver tissues. We found that m⁶A sites with high or very high confidence were present in 39 of these DECs. Four randomly selected DECs were validated by RT-qPCR, hsa-MLIP_0004, hsa-CHD2_0084 and hsa-FOXP1_0001 matched well with the microarray results. m⁶A qualification of them were conducted by MeRIP-qPCR, the m⁶A methylation levels are significantly different between the MAFLD and NC groups.

Conclusion: In MAFLD, the dysregulated expression of circRNAs may be influenced by m⁶A modifications. This study provides preliminary evidence suggesting that m⁶A-mediated regulation of circRNAs could play a role in the progression of MAFLD, laying the foundation for exploring the epigenetic regulation of circRNAs in MAFLD and offering potential avenues for future diagnostic and therapeutic strategies.

Trial registration: Not applicable.

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来源期刊

BMC Gastroenterology 医学-胃肠肝病学

CiteScore

4.20

自引率

0.00%

发文量

465

审稿时长

6 months

期刊介绍： BMC Gastroenterology is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of gastrointestinal and hepatobiliary disorders, as well as related molecular genetics, pathophysiology, and epidemiology.