Mitotic spindle membranes.

Abstract

The mitotic spindle, which uses microtubules (MTs) and MT-based motor proteins to separate sister chromosomes prior to cell division, contains abundant membranes, organelles, and protein assemblies derived from the familiar interphase intracellular membrane network. In this essay, mainly with reference to selected animal and fungal cells, I summarize current ideas about the reciprocal functional relationship between these mitotic spindle-associated membranes and the spindle MT cytoskeleton, in which; 1) spindle membranes control the composition, Ca⁺⁺ ion concentration and mechanical performance of the spindle MT cytoskeleton; and conversely 2) the spindle MT cytoskeleton contributes to membrane/organelle partitioning and inheritance during cell division and serves as a reservoir of membranes, organelles, and vesicles for delivery to the interphase cytoplasm, plasma membrane, and cleavage furrow.