Youhui Jiang , Chuqing Zhou , Jun Zhao , Xinyi Ren , Qiang Wang , Peiyan Ni , Tao Li
{"title":"Derivation of human-derived iPSC line from a male adolescent with first-episode of sporadic schizophrenia","authors":"Youhui Jiang , Chuqing Zhou , Jun Zhao , Xinyi Ren , Qiang Wang , Peiyan Ni , Tao Li","doi":"10.1016/j.scr.2025.103694","DOIUrl":null,"url":null,"abstract":"<div><div>Schizophrenia is considered to be a neurodevelopmental disorder with high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were collected from a male adolescent diagnosed with first-episode of sporadic schizophrenia. Induced pluripotent stem cells (iPSCs) were generated by reprogramming using the factors <em>OCT4</em>, <em>SOX2</em>, <em>NANOG</em>, <em>LIN28</em>, <em>c-MYC</em>, <em>KLF4</em>, and <em>SV40LT</em>. The generated iPSC line was validated by karyotype analysis and expression of pluripotency markers. These iPSCs were capable of differentiating into derivatives of all three germ layers <em>in vivo</em>.</div></div>","PeriodicalId":21843,"journal":{"name":"Stem cell research","volume":"85 ","pages":"Article 103694"},"PeriodicalIF":0.8000,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Stem cell research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1873506125000443","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Schizophrenia is considered to be a neurodevelopmental disorder with high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were collected from a male adolescent diagnosed with first-episode of sporadic schizophrenia. Induced pluripotent stem cells (iPSCs) were generated by reprogramming using the factors OCT4, SOX2, NANOG, LIN28, c-MYC, KLF4, and SV40LT. The generated iPSC line was validated by karyotype analysis and expression of pluripotency markers. These iPSCs were capable of differentiating into derivatives of all three germ layers in vivo.
期刊介绍：
Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
