A Recombinant Vaccinia Virus Expressing a Sodium/Iodide Symporter for Theranostics of Malignant Tumors Using Synchrotron Radiation

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Applied Biochemistry and Microbiology Pub Date : 2025-03-13 DOI:10.1134/S0003683824700273

A. V. Semenova, A. A. Grazhdantseva, G. F. Sivolobova, G. A. Kudrov, A. S. Kabanov, A. A. Legkodymov, K. E. Kuper, A. P. Agafonov, G. V. Kochneva

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Abstract

A recombinant strain of the vaccinia virus, VV-mNIS-dGF, has been constructed. The genome of this strain contains an insertion of the mouse sodium/iodide symporter transgene (mNIS). This insertion is located in the deleted region of the viral thymidine kinase gene (tk). In addition, the viral growth factor gene (vgf) was deleted, ensuring that the virus had an attenuated effect on normal cells without affecting its replication in cancer ones. The antitumor activity of the VV-mNIS-dGF strain in two mouse cancer models, melanoma B16-F10 and colorectal carcinoma CT-26, was studied. In both models, the strain exhibited high oncolytic activity, efficiently expressed the mNIS and accumulated iodide in tumor cells in both in vitro experiments and in vivo on mouse allografts. Quantification of the iodide content in tumors was performed by elemental analysis using X-ray Fluorescence Analysis with Synchrotron Radiation (XRF-SR). The results indicate the possibility of using the VV-mNIS-dGF strain for both therapy and diagnosis (theranostics) of malignant tumors.

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.