Preparation and characterization of a certified reference material of toxic elements in cannabis leaves.

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS Analytical and Bioanalytical Chemistry Pub Date : 2025-03-12 DOI:10.1007/s00216-025-05809-z
Adriana Rodriguez, Cristhian Paredes, Elianna Castillo
{"title":"Preparation and characterization of a certified reference material of toxic elements in cannabis leaves.","authors":"Adriana Rodriguez, Cristhian Paredes, Elianna Castillo","doi":"10.1007/s00216-025-05809-z","DOIUrl":null,"url":null,"abstract":"<p><p>The quantification of harmful elements in cannabis is a relevant analytical task that requires metrological tools to ensure the reliability of the measurement results. This work reports the preparation and characterization of a certified reference material (CRM) for toxic elements-arsenic (As), cadmium (Cd), and lead (Pb)-in pulverized, lyophilized cannabis leaf tissue. To prepare the CRM INM-040-1, a portion of dried ground cannabis vegetal material was spiked with the elements at mass fraction values close to the levels of interest in relevant regulations for this kind of matrix: 0.34 mg/kg for arsenic, 0.34 mg/kg for cadmium, and 0.66 mg/kg for lead. The elements were quantified by inductively coupled plasma mass spectrometry (ICP-MS) in combination with graphite furnace atomic absorption spectroscopy (GF-AAS, for cadmium and lead) or hydride generation atomic absorption spectroscopy (HG-AAS, for arsenic). The analytical calibration was done by gravimetric standard addition for ICP-MS and GF-AAS, while bracketing calibration was used for HG-AAS. Furthermore, internal standard correction was used during ICP-MS measurements. The analytical methods were validated to demonstrate their fitness for purpose. The preparation variables of the CRM (particle size, drying treatment, and spiking conditions) were studied to improve the homogeneity of the CRM. The mass fraction of the toxic elements was certified with relative standard uncertainties ranging from 4.2 to 6.9%. The uncertainty contributions considered were the elements' mass fraction measurements, the between-methods bias, the (in)homogeneity of the production batch, and the (in)stability under transport and storage conditions. This new CRM constitutes a useful tool for the laboratories assessing the harmlessness of cannabis materials, promoting humans' safety and regulatory compliance within the medicinal cannabis industry.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8000,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical and Bioanalytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s00216-025-05809-z","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

The quantification of harmful elements in cannabis is a relevant analytical task that requires metrological tools to ensure the reliability of the measurement results. This work reports the preparation and characterization of a certified reference material (CRM) for toxic elements-arsenic (As), cadmium (Cd), and lead (Pb)-in pulverized, lyophilized cannabis leaf tissue. To prepare the CRM INM-040-1, a portion of dried ground cannabis vegetal material was spiked with the elements at mass fraction values close to the levels of interest in relevant regulations for this kind of matrix: 0.34 mg/kg for arsenic, 0.34 mg/kg for cadmium, and 0.66 mg/kg for lead. The elements were quantified by inductively coupled plasma mass spectrometry (ICP-MS) in combination with graphite furnace atomic absorption spectroscopy (GF-AAS, for cadmium and lead) or hydride generation atomic absorption spectroscopy (HG-AAS, for arsenic). The analytical calibration was done by gravimetric standard addition for ICP-MS and GF-AAS, while bracketing calibration was used for HG-AAS. Furthermore, internal standard correction was used during ICP-MS measurements. The analytical methods were validated to demonstrate their fitness for purpose. The preparation variables of the CRM (particle size, drying treatment, and spiking conditions) were studied to improve the homogeneity of the CRM. The mass fraction of the toxic elements was certified with relative standard uncertainties ranging from 4.2 to 6.9%. The uncertainty contributions considered were the elements' mass fraction measurements, the between-methods bias, the (in)homogeneity of the production batch, and the (in)stability under transport and storage conditions. This new CRM constitutes a useful tool for the laboratories assessing the harmlessness of cannabis materials, promoting humans' safety and regulatory compliance within the medicinal cannabis industry.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
8.00
自引率
4.70%
发文量
638
审稿时长
2.1 months
期刊介绍: Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
期刊最新文献
Isolation of bovine milk-derived extracellular vesicles via a capillary-channeled polymer (C-CP) fiber stationary phase. Assessment of in-house reference materials for multi-pesticide residue analysis in three food matrices by LC-MS/MS: development, homogeneity, and stability. Development of polymer monolith-MOF hybrid via surface functionalization for bioanalytical sciences. Optimizing solvent extraction methods for activated carbon-based passive samplers in atmospheric volatile organic compound analysis: minimizing analytical interferences from pretreatment solvents and ensuring quantitative reliability. Preparation and characterization of a certified reference material of toxic elements in cannabis leaves.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1