Comparatively profiling the transcriptome of human, Porcine and mouse oocytes undergoing meiotic maturation.

IF 3.7 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY BMC Genomics Pub Date : 2025-03-12 DOI:10.1186/s12864-025-11431-1

Naru Zhou, Xin Wang, Yi Xia, Zongliang Liu, Lei Luo, Rentao Jin, Xianhong Tong, Zhenhu Shi, Zhichao Wang, Heming Sui, Yangyang Ma, Yunsheng Li, Zubing Cao, Yunhai Zhang

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Abstract

Background: Oocyte maturation is a critical process responsible for supporting preimplantation embryo development and full development to term. Understanding oocyte gene expression is relevant given the unique molecular mechanism present in this gamete. Comparative transcriptome analysis across species offers a powerful approach to uncover conserved and species-specific genes involved in the molecular regulation of oocyte maturation throughout evolution.

Results: Transcriptome analysis identified 4,625, 3,824, 4,972 differentially expressed genes (DEGs) between the germinal vesicle (GV) and metaphase II (MII) stage in human, porcine and mouse oocytes respectively. These DEGs showed dynamic changes associated with oocyte maturation. Functional enrichment analysis revealed that the DEGs in all three species were mainly involved in DNA replication, cell cycle and redox regulation. Comparative transcriptome analysis identified 551 conserved DEGs in the three species with significant enrichment in mitochondria and mitochondrial intima.

Conclusions: This study provides a systematic comparative analysis of oocyte meiotic maturation in humans, pigs and mice identifying both conserved and species-specific patterns during oocyte meiosis. Our findings also implied that the selection of oocyte expressed genes among these three species could form a basis for further exploring their functional roles in human oocyte maturation.

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人、猪和小鼠卵母细胞减数分裂成熟的转录组比较分析。

背景：卵母细胞成熟是胚胎着床前发育和足月发育的关键过程。考虑到该配子中存在的独特分子机制，了解卵母细胞基因表达是相关的。跨物种的比较转录组分析提供了一种强有力的方法来揭示在整个进化过程中参与卵母细胞成熟分子调控的保守和物种特异性基因。结果：转录组分析在人、猪和小鼠卵母细胞生发囊泡（GV）和中期II （MII）阶段分别鉴定出4,625、3,824和4,972个差异表达基因（deg）。这些deg表现出与卵母细胞成熟相关的动态变化。功能富集分析表明，三种植物的deg主要参与DNA复制、细胞周期和氧化还原调控。比较转录组分析发现，这三个物种中有551个保守的deg，在线粒体和线粒体内膜中显著富集。结论：本研究对人类、猪和小鼠的卵母细胞减数分裂成熟进行了系统的比较分析，确定了卵母细胞减数分裂过程中的保守模式和物种特异性模式。我们的研究结果也表明，这三个物种的卵母细胞表达基因的选择可以为进一步探索它们在人类卵母细胞成熟中的功能作用奠定基础。

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来源期刊

BMC Genomics 生物-生物工程与应用微生物

CiteScore

7.40

自引率

4.50%

发文量

769

审稿时长

6.4 months

期刊介绍： BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.