Exploration of scientific connotation of “Yin-Jing” medical properties of Cyathula officinalis via potentiating therapeutic effect, guidance and targetability

IF 5.4 2区医学 Q1 CHEMISTRY, MEDICINAL Journal of ethnopharmacology Pub Date : 2025-04-25 Epub Date: 2025-03-10 DOI:10.1016/j.jep.2025.119629

Hong-Xiang Jiang , Jun-Hong Chai , Lan Zhou , Xue Gao , Xue-Qing Liu , Wen-Fei Wang , Jun Liang , Hai-Xue Kuang , Yong-Gang Xia

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Abstract

Ethnic pharmacological relevance

“Cyathula officinalis Kuan (COK)” has the effect of “guiding the drug downward” and can enhance the efficacy of formula, e.g., Shentong Zhuyu Decoction (STZYD). However, there is currently no scientific basis on COK to guide drugs to target organs in STZYD.

Aim of the study

The main objective of this study was to unclose the scientific connotations of the Yin-Jing medicinal properties of COK using molecular biology and modern chemical methods.

Materials and methods

A rat model of adjuvant arthritis was established. The optimal dose of STZYD was determined by observing a series of indicators, and the therapeutic effects of STZYD and [STZYD - COK] were compared. The water decoction of COK was divided into five fragments (i.e., Fr. A-E) by macroporous adsorption resin and alcohol deposition methods. The Fr. A-E were further characterized by a combination of multiple chromatographic and spectral techniques. The potentiating therapeutic effects, guidance and targetability tests were used to evaluate “Yin-Jing” function by compatible combination of other drugs using pharmacological indicators, pharmacokinetics, high-performance liquid chromatography (HPLC) and small animal live imaging (SALI) techniques.

Results

The optimal dose of STZYD was confirmed to be 1 × dose and COK increased the efficacy of [STZYD - COK]. The results of chemical characterization showed that the main components of Fr. A-E were polysaccharide, fructooligosaccharide and small M_w fructan, saponins and flavonoid glycosides, steroidal ketones, organic acids esters, respectively. Pharmacological experiments showed that Fr. A, Fr. B and Fr. E were attributed to potentiate therapeutic effects. Guidance assays showed that Fr. B enhanced drug distribution and uptake in the kidneys, joints and cells. Targetability assays further confirmed that Fr. B had apparent targetability toward the joints and kidneys rather than other organs and tissues.

Conclusions

This study for the first time combined potentiating therapeutic effects, guidance and targeting evaluation system, and identified Fr. B as the pharmacodynamic material basis of COK's Yin-Jing medicinal properties.

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从增强疗效、导向性和靶向性探讨牛皮草“阴经”药性的科学内涵。

民族药理学相关性：“宽蹄草”具有“下导药”的作用，可增强方剂的疗效，如参通祛瘀汤。然而，在STZYD中，目前尚无COK指导药物靶向器官的科学依据。研究目的：本研究的主要目的是利用分子生物学和现代化学方法揭示茯苓阴经药性的科学内涵。材料与方法：建立大鼠佐剂性关节炎模型。通过观察一系列指标确定STZYD的最佳剂量，并比较STZYD与[STZYD - COK]的治疗效果。采用大孔吸附树脂法和醇沉法将COK水煎液分成5个片段（即Fr、A-E）。结合多种色谱和光谱技术进一步表征了Fr. a . e。采用药理学指标、药代动力学、高效液相色谱（HPLC）和小动物活体成像（SALI）等技术，对“饮经”与其他药物配用的增强疗效、引导作用和靶向性进行评价。结果：STZYD的最佳剂量为1倍剂量，COK可提高疗效[STZYD-COK]。化学表征结果表明，其主要成分为多糖、低聚果糖和小分子量果聚糖、皂苷和类黄酮苷、甾体酮、有机酸酯。药理实验表明，Fr. A、Fr. B和Fr. E具有增强治疗作用。指导试验显示Fr. B增强了药物在肾脏、关节和细胞中的分布和摄取。靶向性实验进一步证实Fr. B对关节和肾脏而不是其他器官和组织具有明显的靶向性。结论：本研究首次将增效、导效、靶向性评价体系相结合，确定了Fr. B为茯苓阴经药性的药效学物质基础。

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来源期刊

Journal of ethnopharmacology 医学-全科医学与补充医学

CiteScore

10.30

自引率

5.60%

发文量

967

审稿时长

77 days

期刊介绍： The Journal of Ethnopharmacology is dedicated to the exchange of information and understandings about people''s use of plants, fungi, animals, microorganisms and minerals and their biological and pharmacological effects based on the principles established through international conventions. Early people confronted with illness and disease, discovered a wealth of useful therapeutic agents in the plant and animal kingdoms. The empirical knowledge of these medicinal substances and their toxic potential was passed on by oral tradition and sometimes recorded in herbals and other texts on materia medica. Many valuable drugs of today (e.g., atropine, ephedrine, tubocurarine, digoxin, reserpine) came into use through the study of indigenous remedies. Chemists continue to use plant-derived drugs (e.g., morphine, taxol, physostigmine, quinidine, emetine) as prototypes in their attempts to develop more effective and less toxic medicinals.