Development and comparative analysis of clobetasol-loaded microneedle patches versus clobetasol propionate ointment in experimental induced-psoriasis model

Abstract

The utilization of dissolvable microneedles (MNs) is a promising and cutting-edge approach to drug delivery for the treatment of psoriasis, an autoimmune skin disorder characterized by the appearance of red, scaly patches on the skin. This study presents the development of a dissolving MN patch made of polyvinylpyrrolidone for the purpose of delivering Clobetasol 17-Propionate through the skin. The MN patches were evaluated for their physical characteristics, including morphology, solubility, strength, and ability to penetrate the skin. This evaluation was conducted on both unloaded and drug-loaded MN patches to determine their suitability for future applications. The manufacturing of 484 pyramidal-shaped tips, each measuring roughly 400 µm in height, was demonstrated by microscopy photographs. Compression tests revealed that the MN patch could endure a force greater than 1 N/needle while displacing around 300 µm, confirming the needle’s ability to penetrate the stratum corneum. Following H&E staining, the penetration depth in mice skin was determined to be around 200 µm. The MN tips exhibited rapid drug release within a 10-minute timeframe, while the MN patch dissolved in the mice skin in roughly 20 min. An animal model was utilized to examine the effects of the produced patches on the treatment of psoriasis. Psoriasis was artificially induced in three groups of mice using imiquimod cream, applied for eight consecutive days to evaluate the inhibitory effect of clobetasol on exacerbating the disease. This assessment was accomplished using two methods: applying clobetasol ointment and using CP-loaded MNs. This innovative drug delivery system demonstrated encouraging results in terms of quick and effective administration, highlighting the potential of dissolvable MNs for psoriasis treatment.