SubtiToolKit: a bioengineering kit for Bacillus subtilis and Gram-positive bacteria.

IF 14.3 1区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Trends in biotechnology Pub Date : 2025-03-11 DOI:10.1016/j.tibtech.2025.02.004
Joaquin Caro-Astorga, Matt Rogan, Koray Malcı, Hia Ming, Erika Debenedictis, Paul James, Tom Ellis
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引用次数: 0

Abstract

Building DNA constructs of increasing complexity is key to synthetic biology. Golden Gate (GG) methods led to the creation of cloning toolkits - collections of modular standardized DNA parts hosted on hierarchic plasmids, developed for yeast, plants, Gram-negative bacteria, and human cells. However, Gram-positive bacteria have been neglected. Bacillus subtilis is a Gram-positive model organism and a workhorse in the bioindustry. Here, we present the SubtiToolKit (STK), a high-efficiency cloning toolkit for B. subtilis and Gram-positive bacteria. Its design permits DNA constructs for transcriptional units (TUs), operons, and knockin and knockout applications. The STK contains libraries of promoters, ribosome-binding site (RBSs), fluorescent proteins, protein tags, terminators, genome integration parts, a no-leakage genetic device to control the expression of toxic products during Escherichia coli assembly, and a toolbox for industrially relevant strains of Geobacillus and Parageobacillus as an example of the STK versatility for other Gram-positive bacteria and its future perspective as a reference toolkit.

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来源期刊
Trends in biotechnology
Trends in biotechnology 工程技术-生物工程与应用微生物
CiteScore
28.60
自引率
1.20%
发文量
198
审稿时长
1 months
期刊介绍: Trends in Biotechnology publishes reviews and perspectives on the applied biological sciences, focusing on useful science applied to, derived from, or inspired by living systems. The major themes that TIBTECH is interested in include: Bioprocessing (biochemical engineering, applied enzymology, industrial biotechnology, biofuels, metabolic engineering) Omics (genome editing, single-cell technologies, bioinformatics, synthetic biology) Materials and devices (bionanotechnology, biomaterials, diagnostics/imaging/detection, soft robotics, biosensors/bioelectronics) Therapeutics (biofabrication, stem cells, tissue engineering and regenerative medicine, antibodies and other protein drugs, drug delivery) Agroenvironment (environmental engineering, bioremediation, genetically modified crops, sustainable development).
期刊最新文献
Light-induced programmable solid-liquid phase transition of biomolecular condensates for improved biosynthesis. Inducible regulating homologous recombination enables precise genome editing in Pichia pastoris without perturbing cellular fitness. OMEGA-guided DNA polymerases enable random mutagenesis in a tunable window. SubtiToolKit: a bioengineering kit for Bacillus subtilis and Gram-positive bacteria. Engineering biological curvatures.
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