Kuiyu Wang, Yayue Huang, Yu Wan, Wang Chen, Jianwei Liang, Liangke Gong, Zebao Rui, Tao Yang
{"title":"Anti-fouling array paper-based device for rapid and accurate discrimination of foodborne pathogens in real samples","authors":"Kuiyu Wang, Yayue Huang, Yu Wan, Wang Chen, Jianwei Liang, Liangke Gong, Zebao Rui, Tao Yang","doi":"10.1016/j.foodchem.2025.143871","DOIUrl":null,"url":null,"abstract":"Foodborne pathogens contamination can cause serious food safety incidents. Early and rapid detection of foodborne pathogens is very important. Paper-based devices have been applied for on-site rapid detection of foodborne pathogens due to their cheapness and portability. However, the paper-based foodborne pathogen detection devices for real samples often receive interference, resulting in inaccurate detection results. In this study, bovine serum albumin (BSA) was modified on the surface of nitrocellulose membrane to construct anti-fouling sensing interface. Through array design, the anti-fouling array paper-based device (APAD) with multiple detection channels was constructed. The naked eye cut-off limits of the APAD were 10<sup>2</sup> colony forming units (cfu)/mL, 10<sup>2</sup> cfu/mL, and 10<sup>3</sup> cfu/mL for <em>Staphylococcus aureus</em> in orange juice, <em>Escherichia coli</em> O157: H7 in milk and orange juice, and <em>Escherichia coli</em> O157:H7 in soy sauce, respectively. The above results indicate that the APAD has good practical application potential in food safety.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"23 1","pages":""},"PeriodicalIF":8.5000,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.foodchem.2025.143871","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0
Abstract
Foodborne pathogens contamination can cause serious food safety incidents. Early and rapid detection of foodborne pathogens is very important. Paper-based devices have been applied for on-site rapid detection of foodborne pathogens due to their cheapness and portability. However, the paper-based foodborne pathogen detection devices for real samples often receive interference, resulting in inaccurate detection results. In this study, bovine serum albumin (BSA) was modified on the surface of nitrocellulose membrane to construct anti-fouling sensing interface. Through array design, the anti-fouling array paper-based device (APAD) with multiple detection channels was constructed. The naked eye cut-off limits of the APAD were 102 colony forming units (cfu)/mL, 102 cfu/mL, and 103 cfu/mL for Staphylococcus aureus in orange juice, Escherichia coli O157: H7 in milk and orange juice, and Escherichia coli O157:H7 in soy sauce, respectively. The above results indicate that the APAD has good practical application potential in food safety.
期刊介绍:
Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.