Inhibition of PDE-4 and PDE-5 Differentially Modulates Isolated Porcine Urethral Contractility.

IF 1.8 3区 医学 Q3 OBSTETRICS & GYNECOLOGY International Urogynecology Journal Pub Date : 2025-03-13 DOI:10.1007/s00192-025-06102-4
Eriq Burovski, Donna Sellers, Russ Chess-Williams, Iris Lim
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Abstract

Purpose/objective: This study explores the role of phosphodiesterase (PDE) inhibitors (specifically PDE-4, PDE-5 and PDE-1) in modulating the contractility of the porcine urethral smooth muscle and mucosal layers.

Methods: Using an organ bath setup, the effects of PDE inhibitors rolipram, roflumilast, sildenafil, tadalafil and vinpocetine (0.1 nM to 10 μm) on isolated porcine urethral mucosa-intact smooth muscle, mucosa-denuded smooth muscle and mucosal layers were investigated.

Results: Our results demonstrate that PDE-4 inhibitors (rolipram and roflumilast) significantly relaxed mucosa-intact urethral smooth muscle and reduced spontaneous contraction rates in the mucosal strips. Conversely, PDE-5 inhibitors (sildenafil and tadalafil) relaxed smooth muscle tissues denuded of mucosa but required exogenous source of nitric oxide (sodium nitroprusside) for effectiveness in relaxing the mucosa-intact tissues. PDE-1 inhibitor vinpocetine exhibited negligible effects.

Conclusion: The results from the study suggest a potential role of the cAMP pathway in modulating spontaneous contractions within the urethral mucosa, while the NO/cGMP pathway appears to be important in modulating urethral smooth muscle tonic contractions. These findings suggest differential roles of PDE isoenzymes in urethral tissues.

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目的/目标:本研究探讨磷酸二酯酶(PDE)抑制剂(特别是PDE-4、PDE-5和PDE-1)在调节猪尿道平滑肌和粘膜层收缩力中的作用:方法:利用器官浴装置,研究了PDE抑制剂罗利普兰、罗氟米司特、西地那非、他达拉非和长春西汀(0.1 nM至10 μm)对离体猪尿道粘膜-接触平滑肌、粘膜-脱落平滑肌和粘膜层的影响:结果:我们的研究结果表明,PDE-4 抑制剂(罗利普仑和罗氟司特)能显著松弛粘膜接触的尿道平滑肌,并降低粘膜条的自发收缩率。相反,PDE-5 抑制剂(西地那非和他达拉非)可松弛剥离粘膜的平滑肌组织,但需要外源性一氧化氮(硝普钠)才能有效松弛粘膜接触组织。PDE-1抑制剂长春西汀的作用微乎其微:研究结果表明,cAMP 通路在调节尿道粘膜自发收缩中可能发挥作用,而 NO/cGMP 通路在调节尿道平滑肌强直收缩中似乎很重要。这些研究结果表明,PDE 同工酶在尿道组织中发挥着不同的作用。
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来源期刊
CiteScore
3.80
自引率
22.20%
发文量
406
审稿时长
3-6 weeks
期刊介绍: The International Urogynecology Journal is the official journal of the International Urogynecological Association (IUGA).The International Urogynecology Journal has evolved in response to a perceived need amongst the clinicians, scientists, and researchers active in the field of urogynecology and pelvic floor disorders. Gynecologists, urologists, physiotherapists, nurses and basic scientists require regular means of communication within this field of pelvic floor dysfunction to express new ideas and research, and to review clinical practice in the diagnosis and treatment of women with disorders of the pelvic floor. This Journal has adopted the peer review process for all original contributions and will maintain high standards with regard to the research published therein. The clinical approach to urogynecology and pelvic floor disorders will be emphasized with each issue containing clinically relevant material that will be immediately applicable for clinical medicine. This publication covers all aspects of the field in an interdisciplinary fashion
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