Bioprospecting Antarctic Microorganisms: Investigating Hymenobacter psoromatis PAMC26554 for Biochemical Potential.

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of microbiology and biotechnology Pub Date : 2025-03-13 DOI:10.4014/jmb.2412.12010
Hirotake Yamaguchi, Ryoichi Yamada, Jun Hyuck Lee, Tae-Jin Oh
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Abstract

Microorganisms from the genus Hymenobacter are known for their distinctive traits, yet their secondary metabolite (SM) production has not been thoroughly investigated. In this study, we examined the biosynthetic potential of SMs from Hymenobacter psoromatis PAMC26554, which was isolated from Antarctic lichen. In silico analysis identified biosynthetic gene clusters (BGCs) for terpenes, non-ribosomal peptide synthetases (NRPSs), and polyketide synthases (PKSs), indicating the strain's potential for novel SM production. Optimization of culture conditions showed that R2A medium at 15°C supported growth. HPLC analysis revealed phenylacetic acid (PAA) as a notable compound, which was characterized by ESI-MS and NMR, marking the first isolation of PAA from the genus Hymenobacter. In addition, bioactivity assays indicated moderate lipase inhibition by PAA, while molecular docking studies revealed stable interactions with the enzyme, demonstrating that hydrogen bonding and π-π stacking contribute to its lipase inhibitory activity. In summary, this research highlights the genus Hymenobacter as a potential source for secondary metabolite discovery, with PAA exemplifying its unexplored biochemical capabilities.

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来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
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