Critical assessment of quenching and extraction/sample preparation methods for microorganisms in metabolomics.

IF 3.5 3区医学 Q2 ENDOCRINOLOGY & METABOLISM Metabolomics Pub Date : 2025-03-13 DOI:10.1007/s11306-025-02228-0

Hossein Sedighikamal, Shohreh Mashayekhan

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Abstract

Background: Advancements in the research of intracellular metabolome have the potential to affect our understanding of biological processes. The applications and findings of intracellular metabolome analysis are useful in understanding cellular pathways, microbial interactions, and the detection of secreted metabolites and their functions.

Aim of review: This work focuses on the analysis of intracellular metabolomes in microorganisms. The techniques used for analyzing the intracellular metabolomes including metabolomics approaches such as mass spectrometry, nuclear magnetic resonance, liquid chromatography, and gas chromatography are discussed.

Key scientific concepts of review: Challenges such as sample preparation, data analysis, metabolite extraction, sample storage and collection, and processing techniques were investigated, as they can highlight emerging technologies and advancements in metabolome analysis, future applications in drug discovery, personalized medicine, systems biology, and the limitations and challenges in studying the metabolome of microorganisms.

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对代谢组学中微生物的淬火和提取/样品制备方法进行严格评估。

背景：细胞内代谢组研究的进展有可能影响我们对生物过程的理解。细胞内代谢组分析的应用和发现有助于理解细胞途径、微生物相互作用、分泌代谢物及其功能的检测。综述目的：本文主要研究微生物细胞内代谢组的分析。讨论了细胞内代谢组分析技术，包括质谱、核磁共振、液相色谱、气相色谱等代谢组学方法。综述的关键科学概念：研究了样品制备、数据分析、代谢物提取、样品储存和收集以及处理技术等挑战，因为它们可以突出代谢物分析的新兴技术和进展、药物发现、个性化医疗、系统生物学的未来应用以及研究微生物代谢物组的局限性和挑战。

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来源期刊

Metabolomics 医学-内分泌学与代谢

CiteScore

6.60

自引率

2.80%

发文量

审稿时长

2 months

期刊介绍： Metabolomics publishes current research regarding the development of technology platforms for metabolomics. This includes, but is not limited to: metabolomic applications within man, including pre-clinical and clinical pharmacometabolomics for precision medicine metabolic profiling and fingerprinting metabolite target analysis metabolomic applications within animals, plants and microbes transcriptomics and proteomics in systems biology Metabolomics is an indispensable platform for researchers using new post-genomics approaches, to discover networks and interactions between metabolites, pharmaceuticals, SNPs, proteins and more. Its articles go beyond the genome and metabolome, by including original clinical study material together with big data from new emerging technologies.