Alterations to the vaginal microbiota and their correlation with serum pro-inflammatory cytokines in post-weaning sows with endometritis

IF 2.4 2区 农林科学 Q3 REPRODUCTIVE BIOLOGY Theriogenology Pub Date : 2025-03-10 DOI:10.1016/j.theriogenology.2025.117386
Tingting Xian , Yan Liu , Xin Cao , Tao Feng
{"title":"Alterations to the vaginal microbiota and their correlation with serum pro-inflammatory cytokines in post-weaning sows with endometritis","authors":"Tingting Xian ,&nbsp;Yan Liu ,&nbsp;Xin Cao ,&nbsp;Tao Feng","doi":"10.1016/j.theriogenology.2025.117386","DOIUrl":null,"url":null,"abstract":"<div><div>Endometritis is a prevalent disease in sows that strongly reduces reproductive performance. Its causes are complex and multifaceted, making disease management challenging without targeted measures. To provide a scientific basis for developing targeted disease management strategies, we examined vaginal microbiota diversity and its correlation with serum pro-inflammatory cytokine levels in sows with endometritis and the main pathogenic bacterial species contributing to endometritis. Fourteen post-weaning sows selected at a pig farm in Beijing, China, were randomly divided into healthy (C) and endometritis (E) groups. Levels of cytokines including interleukin-1α (IL-1α), IL-1β, IL-6, IL-8, and tumor necrosis factor-α (TNF-α) were quantified by enzyme-linked immunosorbent assays. Vaginal microbiota species were identified using 16S rDNA sequencing. Concentrations of IL-1α and IL-6 in serum and vaginal microbial diversity were significantly increased in group E versus group C. The relative abundance of Firmicutes exhibited a marked increase in group E, whereas that of Fusobacteriota was significantly decreased. The composition and relative abundance of dominant bacterial genera differed between the two groups, and the dominant genera in group E were <em>Clostridium_sensu_stricto_1</em>, <em>UCG-005</em>, <em>UCG-002</em>, <em>Terrisporobacter</em>, and <em>Christensenellaceae_R-7_group</em>. IL-6 was significantly positively correlated with <em>Clostridium_sensu_stricto_1</em> and <em>Terrisporobacter</em> in group E. These results suggest that vaginal microbiota abundance and diversity and serum pro-inflammatory cytokine levels are increased in sows with endometritis, and the latter are closely associated with vaginal microbiota abundance and composition. This study provides a foundation for investigating the pathogenesis of endometritis in sows and a theoretical basis for disease prevention and treatment.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"239 ","pages":"Article 117386"},"PeriodicalIF":2.4000,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Theriogenology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0093691X25001128","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"REPRODUCTIVE BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Endometritis is a prevalent disease in sows that strongly reduces reproductive performance. Its causes are complex and multifaceted, making disease management challenging without targeted measures. To provide a scientific basis for developing targeted disease management strategies, we examined vaginal microbiota diversity and its correlation with serum pro-inflammatory cytokine levels in sows with endometritis and the main pathogenic bacterial species contributing to endometritis. Fourteen post-weaning sows selected at a pig farm in Beijing, China, were randomly divided into healthy (C) and endometritis (E) groups. Levels of cytokines including interleukin-1α (IL-1α), IL-1β, IL-6, IL-8, and tumor necrosis factor-α (TNF-α) were quantified by enzyme-linked immunosorbent assays. Vaginal microbiota species were identified using 16S rDNA sequencing. Concentrations of IL-1α and IL-6 in serum and vaginal microbial diversity were significantly increased in group E versus group C. The relative abundance of Firmicutes exhibited a marked increase in group E, whereas that of Fusobacteriota was significantly decreased. The composition and relative abundance of dominant bacterial genera differed between the two groups, and the dominant genera in group E were Clostridium_sensu_stricto_1, UCG-005, UCG-002, Terrisporobacter, and Christensenellaceae_R-7_group. IL-6 was significantly positively correlated with Clostridium_sensu_stricto_1 and Terrisporobacter in group E. These results suggest that vaginal microbiota abundance and diversity and serum pro-inflammatory cytokine levels are increased in sows with endometritis, and the latter are closely associated with vaginal microbiota abundance and composition. This study provides a foundation for investigating the pathogenesis of endometritis in sows and a theoretical basis for disease prevention and treatment.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
子宫内膜炎是母猪的一种常见病,会严重降低繁殖性能。子宫内膜炎的病因复杂且涉及多个方面,如果不采取有针对性的措施,就很难控制疾病。为了给制定有针对性的疾病管理策略提供科学依据,我们研究了子宫内膜炎母猪阴道微生物群的多样性及其与血清促炎细胞因子水平的相关性,以及导致子宫内膜炎的主要致病菌种类。在中国北京的一个养猪场选取 14 头断奶后母猪,随机分为健康组(C)和子宫内膜炎组(E)。用酶联免疫吸附试验定量检测白细胞介素-1α(IL-1α)、IL-1β、IL-6、IL-8和肿瘤坏死因子-α(TNF-α)等细胞因子的水平。使用 16S rDNA 测序鉴定了阴道微生物群的种类。与 C 组相比,E 组血清和阴道微生物多样性中 IL-1α 和 IL-6 的浓度显著增加。两组优势菌属的组成和相对丰度存在差异,E 组的优势菌属为梭菌_sensu_stricto_1、UCG-005、UCG-002、Terrisporobacter 和 Christensenellaceae_R-7_group。这些结果表明,患有子宫内膜炎的母猪阴道微生物群的丰度和多样性以及血清促炎细胞因子水平都有所增加,而后者与阴道微生物群的丰度和组成密切相关。这项研究为研究母猪子宫内膜炎的发病机制奠定了基础,也为疾病的预防和治疗提供了理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Theriogenology
Theriogenology 农林科学-生殖生物学
CiteScore
5.50
自引率
14.30%
发文量
387
审稿时长
72 days
期刊介绍: Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.
期刊最新文献
Fertility preservation of vacuum-dried ram spermatozoa stored for four years at room temperature 5-Aminolevulinate acid improves boar semen quality by enhancing the sperm mitochondrial function Editorial Board Editorial Board Mangiferin promotes porcine oocyte maturation and delays the postovulatory aging process by up-regulating NRF2 levels
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1