Alberto Izquierdo Martínez, Carmen Sánchez-Palacios
{"title":"IMPORTANCE OF THE PREANALYTICAL PHASE: ELEVATION OF PROTEIN LEVELS IN CEREBROSPINAL FLUID SAMPLES DUE TO INAPPROPRIATE CONTAINERS.","authors":"Alberto Izquierdo Martínez, Carmen Sánchez-Palacios","doi":"10.1177/00045632251330749","DOIUrl":null,"url":null,"abstract":"<p><p>Biomarkers in cerebrospinal fluid (CSF) are crucial for diagnosing, monitoring, and prognosing neurological disorders. This study evaluates the impact of preanalytical variables, particularly container choice, on CSF protein measurements. Using 30 CSF samples, we compared sterile, additive-free tubes and lithium heparin tubes without separator gel. Protein levels were significantly elevated higher in heparin tubes (mean difference: 230.71 mg/dL, p<0.001). This overestimation underscores the necessity of adhering to preanalytical protocols to avoid erroneous clinical interpretations and ensure accurate diagnostic outcomes.</p>","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"45632251330749"},"PeriodicalIF":2.1000,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of Clinical Biochemistry","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/00045632251330749","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Biomarkers in cerebrospinal fluid (CSF) are crucial for diagnosing, monitoring, and prognosing neurological disorders. This study evaluates the impact of preanalytical variables, particularly container choice, on CSF protein measurements. Using 30 CSF samples, we compared sterile, additive-free tubes and lithium heparin tubes without separator gel. Protein levels were significantly elevated higher in heparin tubes (mean difference: 230.71 mg/dL, p<0.001). This overestimation underscores the necessity of adhering to preanalytical protocols to avoid erroneous clinical interpretations and ensure accurate diagnostic outcomes.
期刊介绍：
Annals of Clinical Biochemistry is the fully peer reviewed international journal of the Association for Clinical Biochemistry and Laboratory Medicine.
Annals of Clinical Biochemistry accepts papers that contribute to knowledge in all fields of laboratory medicine, especially those pertaining to the understanding, diagnosis and treatment of human disease. It publishes papers on clinical biochemistry, clinical audit, metabolic medicine, immunology, genetics, biotechnology, haematology, microbiology, computing and management where they have both biochemical and clinical relevance. Papers describing evaluation or implementation of commercial reagent kits or the performance of new analysers require substantial original information. Unless of exceptional interest and novelty, studies dealing with the redox status in various diseases are not generally considered within the journal''s scope. Studies documenting the association of single nucleotide polymorphisms (SNPs) with particular phenotypes will not normally be considered, given the greater strength of genome wide association studies (GWAS). Research undertaken in non-human animals will not be considered for publication in the Annals.
Annals of Clinical Biochemistry is also the official journal of NVKC (de Nederlandse Vereniging voor Klinische Chemie) and JSCC (Japan Society of Clinical Chemistry).
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
